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Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles

CRISPR-Cas12a is a leading technology for development of model organisms, therapeutics, and diagnostics. These applications could benefit from chemical modifications that stabilize or tune enzyme properties. Here we chemically modify ribonucleotides of the AsCas12a CRISPR RNA 5′ handle, a pseudoknot...

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Detalles Bibliográficos
Autores principales:	Ageely, Eman A., Chilamkurthy, Ramadevi, Jana, Sunit, Abdullahu, Leonora, O’Reilly, Daniel, Jensik, Philip J., Damha, Masad J., Gagnon, Keith T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593028/ https://www.ncbi.nlm.nih.gov/pubmed/34782635 http://dx.doi.org/10.1038/s41467-021-26989-z

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