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Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes

The purpose of this study was to characterize the alterations in microstructural organization of arterial tissue using higher-order diffusion magnetic resonance schemes. Three porcine carotid artery models namely; native, collagenase treated and decellularized, were used to estimate the contribution...

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Autores principales: Shahid, Syed Salman, Johnston, Robert D., Smekens, Celine, Kerskens, Christian, Gaul, Robert, Tornifoglio, Brooke, Stone, Alan J., Lally, Caitríona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593063/
https://www.ncbi.nlm.nih.gov/pubmed/34782651
http://dx.doi.org/10.1038/s41598-021-01476-z
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author Shahid, Syed Salman
Johnston, Robert D.
Smekens, Celine
Kerskens, Christian
Gaul, Robert
Tornifoglio, Brooke
Stone, Alan J.
Lally, Caitríona
author_facet Shahid, Syed Salman
Johnston, Robert D.
Smekens, Celine
Kerskens, Christian
Gaul, Robert
Tornifoglio, Brooke
Stone, Alan J.
Lally, Caitríona
author_sort Shahid, Syed Salman
collection PubMed
description The purpose of this study was to characterize the alterations in microstructural organization of arterial tissue using higher-order diffusion magnetic resonance schemes. Three porcine carotid artery models namely; native, collagenase treated and decellularized, were used to estimate the contribution of collagen and smooth muscle cells (SMC) on diffusion signal attenuation using gaussian and non-gaussian schemes. The samples were imaged in a 7 T preclinical scanner. High spatial and angular resolution diffusion weighted images (DWIs) were acquired using two multi-shell (max b-value = 3000 s/mm(2)) acquisition protocols. The processed DWIs were fitted using monoexponential, stretched-exponential, kurtosis and bi-exponential schemes. Directionally variant and invariant microstructural parametric maps of the three artery models were obtained from the diffusion schemes. The parametric maps were used to assess the sensitivity of each diffusion scheme to collagen and SMC composition in arterial microstructural environment. The inter-model comparison showed significant differences across the considered models. The bi-exponential scheme based slow diffusion compartment (Ds) was highest in the absence of collagen, compared to native and decellularized microenvironments. In intra-model comparison, kurtosis along the radial direction was the highest. Overall, the results of this study demonstrate the efficacy of higher order dMRI schemes in mapping constituent specific alterations in arterial microstructure.
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spelling pubmed-85930632021-11-16 Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes Shahid, Syed Salman Johnston, Robert D. Smekens, Celine Kerskens, Christian Gaul, Robert Tornifoglio, Brooke Stone, Alan J. Lally, Caitríona Sci Rep Article The purpose of this study was to characterize the alterations in microstructural organization of arterial tissue using higher-order diffusion magnetic resonance schemes. Three porcine carotid artery models namely; native, collagenase treated and decellularized, were used to estimate the contribution of collagen and smooth muscle cells (SMC) on diffusion signal attenuation using gaussian and non-gaussian schemes. The samples were imaged in a 7 T preclinical scanner. High spatial and angular resolution diffusion weighted images (DWIs) were acquired using two multi-shell (max b-value = 3000 s/mm(2)) acquisition protocols. The processed DWIs were fitted using monoexponential, stretched-exponential, kurtosis and bi-exponential schemes. Directionally variant and invariant microstructural parametric maps of the three artery models were obtained from the diffusion schemes. The parametric maps were used to assess the sensitivity of each diffusion scheme to collagen and SMC composition in arterial microstructural environment. The inter-model comparison showed significant differences across the considered models. The bi-exponential scheme based slow diffusion compartment (Ds) was highest in the absence of collagen, compared to native and decellularized microenvironments. In intra-model comparison, kurtosis along the radial direction was the highest. Overall, the results of this study demonstrate the efficacy of higher order dMRI schemes in mapping constituent specific alterations in arterial microstructure. Nature Publishing Group UK 2021-11-15 /pmc/articles/PMC8593063/ /pubmed/34782651 http://dx.doi.org/10.1038/s41598-021-01476-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shahid, Syed Salman
Johnston, Robert D.
Smekens, Celine
Kerskens, Christian
Gaul, Robert
Tornifoglio, Brooke
Stone, Alan J.
Lally, Caitríona
Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
title Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
title_full Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
title_fullStr Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
title_full_unstemmed Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
title_short Exploring arterial tissue microstructural organization using non-Gaussian diffusion magnetic resonance schemes
title_sort exploring arterial tissue microstructural organization using non-gaussian diffusion magnetic resonance schemes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593063/
https://www.ncbi.nlm.nih.gov/pubmed/34782651
http://dx.doi.org/10.1038/s41598-021-01476-z
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