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Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression

Long non-coding RNAs (lncRNAs) serve essential roles on various biological functions. Previous studies have indicated that lncRNAs are involved in the occurrence, growth and infiltration of brain tumors. LncRNA H19 is key regulator in the pathogenesis of gliomas, but the underlying mechanisms of H19...

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Autores principales: Chen, Xuezhu, Li, Yuhong, Zuo, Chenghai, Zhang, Kaiyuan, Lei, Xuejiao, Wang, Ju, Yang, Yang, Zhang, Jianmin, Ma, Kang, Wang, Shi, Mu, Ning, Yang, Chuanyan, Xian, Jishu, Feng, Hua, Tang, Rongrui, Chen, Tunan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593200/
https://www.ncbi.nlm.nih.gov/pubmed/34796112
http://dx.doi.org/10.3389/fonc.2021.757650
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author Chen, Xuezhu
Li, Yuhong
Zuo, Chenghai
Zhang, Kaiyuan
Lei, Xuejiao
Wang, Ju
Yang, Yang
Zhang, Jianmin
Ma, Kang
Wang, Shi
Mu, Ning
Yang, Chuanyan
Xian, Jishu
Feng, Hua
Tang, Rongrui
Chen, Tunan
author_facet Chen, Xuezhu
Li, Yuhong
Zuo, Chenghai
Zhang, Kaiyuan
Lei, Xuejiao
Wang, Ju
Yang, Yang
Zhang, Jianmin
Ma, Kang
Wang, Shi
Mu, Ning
Yang, Chuanyan
Xian, Jishu
Feng, Hua
Tang, Rongrui
Chen, Tunan
author_sort Chen, Xuezhu
collection PubMed
description Long non-coding RNAs (lncRNAs) serve essential roles on various biological functions. Previous studies have indicated that lncRNAs are involved in the occurrence, growth and infiltration of brain tumors. LncRNA H19 is key regulator in the pathogenesis of gliomas, but the underlying mechanisms of H19-regulated tumor progression remain unknown. Therefore, we investigated the effects and mechanism of action of lncRNA H19 on the homeostasis of glioma cells. As a novel oncogenic factor, up-regulation of H19 was able to promote the proliferation of glioma cells by targeting miR-200a. Furthermore, elevated miR-200a levels could reverse H19-induced cell growth and metastasis. Overexpression of miR-200a could significantly suppress the proliferation, migration and invasion of glioma cells. These biological behavior changes in glioma cells were dependent on the binding to potential target genes including CDK6 and ZEB1. CDK6 could promote cell proliferation and its expression was remarkably increased in glioma. In addition, up-regulation of miR-200a lead to reduction of CDK6 expression and inhibit the proliferation of glioma cells. ZEB1 could be a putative target gene of miR-200a in glioma cells. Thus, miR-200a might suppress cell invasion and migration through down-regulating ZEB1. Moreover, overexpression of miR-200a resulted in down-regulation of ZEB1 and further inhibited malignant phenotype of glioma cells. In summary, our findings suggested that the expression of H19 was elevated in glioma, which could promote the growth, invasion and migration of tumor cells via H19/miR-200a/CDK6/ZEB1 axis. This novel signaling pathway may be a promising candidate for the diagnosis and targeted treatment of glioma.
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spelling pubmed-85932002021-11-17 Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression Chen, Xuezhu Li, Yuhong Zuo, Chenghai Zhang, Kaiyuan Lei, Xuejiao Wang, Ju Yang, Yang Zhang, Jianmin Ma, Kang Wang, Shi Mu, Ning Yang, Chuanyan Xian, Jishu Feng, Hua Tang, Rongrui Chen, Tunan Front Oncol Oncology Long non-coding RNAs (lncRNAs) serve essential roles on various biological functions. Previous studies have indicated that lncRNAs are involved in the occurrence, growth and infiltration of brain tumors. LncRNA H19 is key regulator in the pathogenesis of gliomas, but the underlying mechanisms of H19-regulated tumor progression remain unknown. Therefore, we investigated the effects and mechanism of action of lncRNA H19 on the homeostasis of glioma cells. As a novel oncogenic factor, up-regulation of H19 was able to promote the proliferation of glioma cells by targeting miR-200a. Furthermore, elevated miR-200a levels could reverse H19-induced cell growth and metastasis. Overexpression of miR-200a could significantly suppress the proliferation, migration and invasion of glioma cells. These biological behavior changes in glioma cells were dependent on the binding to potential target genes including CDK6 and ZEB1. CDK6 could promote cell proliferation and its expression was remarkably increased in glioma. In addition, up-regulation of miR-200a lead to reduction of CDK6 expression and inhibit the proliferation of glioma cells. ZEB1 could be a putative target gene of miR-200a in glioma cells. Thus, miR-200a might suppress cell invasion and migration through down-regulating ZEB1. Moreover, overexpression of miR-200a resulted in down-regulation of ZEB1 and further inhibited malignant phenotype of glioma cells. In summary, our findings suggested that the expression of H19 was elevated in glioma, which could promote the growth, invasion and migration of tumor cells via H19/miR-200a/CDK6/ZEB1 axis. This novel signaling pathway may be a promising candidate for the diagnosis and targeted treatment of glioma. Frontiers Media S.A. 2021-11-02 /pmc/articles/PMC8593200/ /pubmed/34796112 http://dx.doi.org/10.3389/fonc.2021.757650 Text en Copyright © 2021 Chen, Li, Zuo, Zhang, Lei, Wang, Yang, Zhang, Ma, Wang, Mu, Yang, Xian, Feng, Tang and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Chen, Xuezhu
Li, Yuhong
Zuo, Chenghai
Zhang, Kaiyuan
Lei, Xuejiao
Wang, Ju
Yang, Yang
Zhang, Jianmin
Ma, Kang
Wang, Shi
Mu, Ning
Yang, Chuanyan
Xian, Jishu
Feng, Hua
Tang, Rongrui
Chen, Tunan
Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression
title Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression
title_full Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression
title_fullStr Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression
title_full_unstemmed Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression
title_short Long Non−Coding RNA H19 Regulates Glioma Cell Growth and Metastasis via miR-200a-Mediated CDK6 and ZEB1 Expression
title_sort long non−coding rna h19 regulates glioma cell growth and metastasis via mir-200a-mediated cdk6 and zeb1 expression
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593200/
https://www.ncbi.nlm.nih.gov/pubmed/34796112
http://dx.doi.org/10.3389/fonc.2021.757650
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