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Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit
OBJECTIVE: To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. METHODS: Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells wer...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593314/ https://www.ncbi.nlm.nih.gov/pubmed/34775864 http://dx.doi.org/10.1177/03000605211055384 |
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author | Huang, Sheng Xu, Kangli Xu, Shanshan Yu, Shijun Miao, Yan Wang, Juan |
author_facet | Huang, Sheng Xu, Kangli Xu, Shanshan Yu, Shijun Miao, Yan Wang, Juan |
author_sort | Huang, Sheng |
collection | PubMed |
description | OBJECTIVE: To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. METHODS: Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software. RESULTS: A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively. CONCLUSIONS: The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples. |
format | Online Article Text |
id | pubmed-8593314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-85933142021-11-17 Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit Huang, Sheng Xu, Kangli Xu, Shanshan Yu, Shijun Miao, Yan Wang, Juan J Int Med Res Prospective Clinical Research Report OBJECTIVE: To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. METHODS: Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software. RESULTS: A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively. CONCLUSIONS: The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples. SAGE Publications 2021-11-13 /pmc/articles/PMC8593314/ /pubmed/34775864 http://dx.doi.org/10.1177/03000605211055384 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Prospective Clinical Research Report Huang, Sheng Xu, Kangli Xu, Shanshan Yu, Shijun Miao, Yan Wang, Juan Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
title | Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
title_full | Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
title_fullStr | Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
title_full_unstemmed | Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
title_short | Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit |
title_sort | enumeration of viable cd34+ cells in cord blood using a novel stem cell enumeration kit |
topic | Prospective Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593314/ https://www.ncbi.nlm.nih.gov/pubmed/34775864 http://dx.doi.org/10.1177/03000605211055384 |
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