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Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production

Yeasts are alternative source of natural carotenoids, a group of colored terpenoids with various market applications. Carotenoid Production by yeast fermentation technology is greatly effective and proposes considerable benefits with large scale production, cost effectiveness and safety. In this stu...

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Autores principales: Allahkarami, Somayeh, Sepahi, Abbas Akhavan, Hosseini, Hedayat, Razavi, Mohamad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593566/
https://www.ncbi.nlm.nih.gov/pubmed/34815952
http://dx.doi.org/10.1016/j.btre.2021.e00687
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author Allahkarami, Somayeh
Sepahi, Abbas Akhavan
Hosseini, Hedayat
Razavi, Mohamad Reza
author_facet Allahkarami, Somayeh
Sepahi, Abbas Akhavan
Hosseini, Hedayat
Razavi, Mohamad Reza
author_sort Allahkarami, Somayeh
collection PubMed
description Yeasts are alternative source of natural carotenoids, a group of colored terpenoids with various market applications. Carotenoid Production by yeast fermentation technology is greatly effective and proposes considerable benefits with large scale production, cost effectiveness and safety. In this study, four pigment-producing yeasts were isolated from forest park soils with the potential to produce carotenoids. Morphological, physiological, biochemical and molecular characterization indicates the isolates belong to Rhodotorula mucilaginosa. Carotenoid production was optimized by small scale cultivation. The optimum condition was 120 h of incubation at pH 6.0, 28 °C, white light irradiation, Yeast Extract Peptone Glycerol medium composed of 10 g/L yeast extract, 20 g/L peptone, 20 ml/L glycerol, yielding maximum content of 223.5 μg/g dry weight. The β-carotene content was confirmed by HPLC and FT-IR. The results suggested that soil yeasts are potential sources of carotenoids that could be utilized as a natural agent for industrial products.
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spelling pubmed-85935662021-11-22 Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production Allahkarami, Somayeh Sepahi, Abbas Akhavan Hosseini, Hedayat Razavi, Mohamad Reza Biotechnol Rep (Amst) Research Article Yeasts are alternative source of natural carotenoids, a group of colored terpenoids with various market applications. Carotenoid Production by yeast fermentation technology is greatly effective and proposes considerable benefits with large scale production, cost effectiveness and safety. In this study, four pigment-producing yeasts were isolated from forest park soils with the potential to produce carotenoids. Morphological, physiological, biochemical and molecular characterization indicates the isolates belong to Rhodotorula mucilaginosa. Carotenoid production was optimized by small scale cultivation. The optimum condition was 120 h of incubation at pH 6.0, 28 °C, white light irradiation, Yeast Extract Peptone Glycerol medium composed of 10 g/L yeast extract, 20 g/L peptone, 20 ml/L glycerol, yielding maximum content of 223.5 μg/g dry weight. The β-carotene content was confirmed by HPLC and FT-IR. The results suggested that soil yeasts are potential sources of carotenoids that could be utilized as a natural agent for industrial products. Elsevier 2021-11-03 /pmc/articles/PMC8593566/ /pubmed/34815952 http://dx.doi.org/10.1016/j.btre.2021.e00687 Text en © 2021 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Allahkarami, Somayeh
Sepahi, Abbas Akhavan
Hosseini, Hedayat
Razavi, Mohamad Reza
Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production
title Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production
title_full Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production
title_fullStr Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production
title_full_unstemmed Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production
title_short Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production
title_sort isolation and identification of carotenoid-producing rhodotorula sp. from pinaceae forest ecosystems and optimization of in vitro carotenoid production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593566/
https://www.ncbi.nlm.nih.gov/pubmed/34815952
http://dx.doi.org/10.1016/j.btre.2021.e00687
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