A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice

Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide...

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Detalles Bibliográficos
Autores principales: Nolan, Lila S., Gong, Qingqing, Hofmeister, Heather N., Good, Misty
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593655/
https://www.ncbi.nlm.nih.gov/pubmed/34816129
http://dx.doi.org/10.1016/j.xpro.2021.100951
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author Nolan, Lila S.
Gong, Qingqing
Hofmeister, Heather N.
Good, Misty
author_facet Nolan, Lila S.
Gong, Qingqing
Hofmeister, Heather N.
Good, Misty
author_sort Nolan, Lila S.
collection PubMed
description Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).
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spelling pubmed-85936552021-11-22 A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice Nolan, Lila S. Gong, Qingqing Hofmeister, Heather N. Good, Misty STAR Protoc Protocol Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021). Elsevier 2021-11-11 /pmc/articles/PMC8593655/ /pubmed/34816129 http://dx.doi.org/10.1016/j.xpro.2021.100951 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nolan, Lila S.
Gong, Qingqing
Hofmeister, Heather N.
Good, Misty
A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
title A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
title_full A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
title_fullStr A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
title_full_unstemmed A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
title_short A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
title_sort protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593655/
https://www.ncbi.nlm.nih.gov/pubmed/34816129
http://dx.doi.org/10.1016/j.xpro.2021.100951
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