Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on e...

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Detalles Bibliográficos
Autores principales: Stappers, Mark H.T., Nikolakopoulou, Christina, Wiesner, Darin L., Yuecel, Raif, Klein, Bruce S., Willment, Janet A., Brown, Gordon D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Technical Comment
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593890/
https://www.ncbi.nlm.nih.gov/pubmed/34114658
http://dx.doi.org/10.1002/eji.202149192
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author Stappers, Mark H.T.
Nikolakopoulou, Christina
Wiesner, Darin L.
Yuecel, Raif
Klein, Bruce S.
Willment, Janet A.
Brown, Gordon D.
author_facet Stappers, Mark H.T.
Nikolakopoulou, Christina
Wiesner, Darin L.
Yuecel, Raif
Klein, Bruce S.
Willment, Janet A.
Brown, Gordon D.
author_sort Stappers, Mark H.T.
collection PubMed
description Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues. [Image: see text]
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spelling pubmed-85938902021-11-22 Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues Stappers, Mark H.T. Nikolakopoulou, Christina Wiesner, Darin L. Yuecel, Raif Klein, Bruce S. Willment, Janet A. Brown, Gordon D. Eur J Immunol Technical Comment Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues. [Image: see text] John Wiley and Sons Inc. 2021-06-23 2021-09 /pmc/articles/PMC8593890/ /pubmed/34114658 http://dx.doi.org/10.1002/eji.202149192 Text en © 2021 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Comment
Stappers, Mark H.T.
Nikolakopoulou, Christina
Wiesner, Darin L.
Yuecel, Raif
Klein, Bruce S.
Willment, Janet A.
Brown, Gordon D.
Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
title Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
title_full Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
title_fullStr Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
title_full_unstemmed Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
title_short Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
title_sort characterization of antifungal c‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
topic Technical Comment
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593890/
https://www.ncbi.nlm.nih.gov/pubmed/34114658
http://dx.doi.org/10.1002/eji.202149192
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