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Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
While molecular assays, such as reverse-transcription polymerase chain reaction (RT-PCR), have been widely used throughout the coronavirus disease 2019 (COVID-19) pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing u...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594033/ https://www.ncbi.nlm.nih.gov/pubmed/34851326 http://dx.doi.org/10.3389/fitd.2021.707865 |
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author | Salcedo, Nol Harmon, Alexander Herrera, Bobby Brooke |
author_facet | Salcedo, Nol Harmon, Alexander Herrera, Bobby Brooke |
author_sort | Salcedo, Nol |
collection | PubMed |
description | While molecular assays, such as reverse-transcription polymerase chain reaction (RT-PCR), have been widely used throughout the coronavirus disease 2019 (COVID-19) pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing using RT-PCR has been implemented. However, pooling samples for antigen testing has not been evaluated. Here, we propose a proof-of-concept pooling strategy for antigen testing that would significantly expand SARS-CoV-2 surveillance, especially for low-to-middle income countries, schools, and workplaces. Our laboratory-based testing demonstrates that combining of up to 20 nasal swab specimens per pool can expand surveillance with antigen tests, even if a pool contains only one positive sample. |
format | Online Article Text |
id | pubmed-8594033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85940332021-11-16 Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test Salcedo, Nol Harmon, Alexander Herrera, Bobby Brooke Front Trop Dis Tropical Diseases While molecular assays, such as reverse-transcription polymerase chain reaction (RT-PCR), have been widely used throughout the coronavirus disease 2019 (COVID-19) pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing using RT-PCR has been implemented. However, pooling samples for antigen testing has not been evaluated. Here, we propose a proof-of-concept pooling strategy for antigen testing that would significantly expand SARS-CoV-2 surveillance, especially for low-to-middle income countries, schools, and workplaces. Our laboratory-based testing demonstrates that combining of up to 20 nasal swab specimens per pool can expand surveillance with antigen tests, even if a pool contains only one positive sample. Frontiers Media S.A. 2021-08-05 /pmc/articles/PMC8594033/ /pubmed/34851326 http://dx.doi.org/10.3389/fitd.2021.707865 Text en Copyright © 2021 Salcedo, Harmon and Herrera https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Tropical Diseases Salcedo, Nol Harmon, Alexander Herrera, Bobby Brooke Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test |
title | Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test |
title_full | Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test |
title_fullStr | Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test |
title_full_unstemmed | Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test |
title_short | Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test |
title_sort | pooling of samples for sars-cov-2 detection using a rapid antigen test |
topic | Tropical Diseases |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594033/ https://www.ncbi.nlm.nih.gov/pubmed/34851326 http://dx.doi.org/10.3389/fitd.2021.707865 |
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