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Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test

While molecular assays, such as reverse-transcription polymerase chain reaction (RT-PCR), have been widely used throughout the coronavirus disease 2019 (COVID-19) pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing u...

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Detalles Bibliográficos
Autores principales: Salcedo, Nol, Harmon, Alexander, Herrera, Bobby Brooke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594033/
https://www.ncbi.nlm.nih.gov/pubmed/34851326
http://dx.doi.org/10.3389/fitd.2021.707865
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author Salcedo, Nol
Harmon, Alexander
Herrera, Bobby Brooke
author_facet Salcedo, Nol
Harmon, Alexander
Herrera, Bobby Brooke
author_sort Salcedo, Nol
collection PubMed
description While molecular assays, such as reverse-transcription polymerase chain reaction (RT-PCR), have been widely used throughout the coronavirus disease 2019 (COVID-19) pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing using RT-PCR has been implemented. However, pooling samples for antigen testing has not been evaluated. Here, we propose a proof-of-concept pooling strategy for antigen testing that would significantly expand SARS-CoV-2 surveillance, especially for low-to-middle income countries, schools, and workplaces. Our laboratory-based testing demonstrates that combining of up to 20 nasal swab specimens per pool can expand surveillance with antigen tests, even if a pool contains only one positive sample.
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spelling pubmed-85940332021-11-16 Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test Salcedo, Nol Harmon, Alexander Herrera, Bobby Brooke Front Trop Dis Tropical Diseases While molecular assays, such as reverse-transcription polymerase chain reaction (RT-PCR), have been widely used throughout the coronavirus disease 2019 (COVID-19) pandemic, the technique is costly and resource intensive. As a means to reduce costs and increase diagnostic efficiency, pooled testing using RT-PCR has been implemented. However, pooling samples for antigen testing has not been evaluated. Here, we propose a proof-of-concept pooling strategy for antigen testing that would significantly expand SARS-CoV-2 surveillance, especially for low-to-middle income countries, schools, and workplaces. Our laboratory-based testing demonstrates that combining of up to 20 nasal swab specimens per pool can expand surveillance with antigen tests, even if a pool contains only one positive sample. Frontiers Media S.A. 2021-08-05 /pmc/articles/PMC8594033/ /pubmed/34851326 http://dx.doi.org/10.3389/fitd.2021.707865 Text en Copyright © 2021 Salcedo, Harmon and Herrera https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Tropical Diseases
Salcedo, Nol
Harmon, Alexander
Herrera, Bobby Brooke
Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
title Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
title_full Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
title_fullStr Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
title_full_unstemmed Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
title_short Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test
title_sort pooling of samples for sars-cov-2 detection using a rapid antigen test
topic Tropical Diseases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594033/
https://www.ncbi.nlm.nih.gov/pubmed/34851326
http://dx.doi.org/10.3389/fitd.2021.707865
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