Cargando…

Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats

BACKGROUND: Gentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in rats. Thi...

Descripción completa

Detalles Bibliográficos
Autores principales: Abouzed, Tarek Kamal, Sherif, Eman Abd Elrahman, Barakat, Mohamed El Sayed, Sadek, Kadry Mohamed, Aldhahrani, Adil, Nasr, Nasr Elsayed, Eldomany, Ehab, Khailo, Khaled, Dorghamm, Doaa Abdallha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594120/
https://www.ncbi.nlm.nih.gov/pubmed/34784920
http://dx.doi.org/10.1186/s12917-021-03023-4
_version_ 1784599902817353728
author Abouzed, Tarek Kamal
Sherif, Eman Abd Elrahman
Barakat, Mohamed El Sayed
Sadek, Kadry Mohamed
Aldhahrani, Adil
Nasr, Nasr Elsayed
Eldomany, Ehab
Khailo, Khaled
Dorghamm, Doaa Abdallha
author_facet Abouzed, Tarek Kamal
Sherif, Eman Abd Elrahman
Barakat, Mohamed El Sayed
Sadek, Kadry Mohamed
Aldhahrani, Adil
Nasr, Nasr Elsayed
Eldomany, Ehab
Khailo, Khaled
Dorghamm, Doaa Abdallha
author_sort Abouzed, Tarek Kamal
collection PubMed
description BACKGROUND: Gentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in rats. Thirty Wistar rats were divided into three groups of 10: a control group, which received no treatment; a gentamicin group administered by a dose of (100 mg/kg, IP) for 7 consecutive days, and a cisplatin group was administered intraperitoneal in a dose of (1.5 mg/kg body weight) repeated twice a week for 3 weeks. RESULTS: Both experimental groups exhibited increased levels of creatinine, urea, and uric acid, with the cisplatin-treated group showing higher levels than the gentamicin group. Experimental groups also exhibited significantly increased Malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GSH-Px) with more pronounced effects in the cisplatin-treated group. Further, both experimental groups exhibited significant up-regulation of Tumor Necrosis Factor α (TNF-α), caspase-3, and Bax and down regulation of Bcl-2. CONCLUSION: These findings confirm the use of necrotic, apoptotic genes as early biomarkers in the detection of tubular kidney damage. Further, cisplatin was shown to have a greater nephrotoxic effect than gentamicin; therefore, its use should be constrained accordingly when co-administered with gentamicin.
format Online
Article
Text
id pubmed-8594120
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-85941202021-11-16 Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats Abouzed, Tarek Kamal Sherif, Eman Abd Elrahman Barakat, Mohamed El Sayed Sadek, Kadry Mohamed Aldhahrani, Adil Nasr, Nasr Elsayed Eldomany, Ehab Khailo, Khaled Dorghamm, Doaa Abdallha BMC Vet Res Research Article BACKGROUND: Gentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in rats. Thirty Wistar rats were divided into three groups of 10: a control group, which received no treatment; a gentamicin group administered by a dose of (100 mg/kg, IP) for 7 consecutive days, and a cisplatin group was administered intraperitoneal in a dose of (1.5 mg/kg body weight) repeated twice a week for 3 weeks. RESULTS: Both experimental groups exhibited increased levels of creatinine, urea, and uric acid, with the cisplatin-treated group showing higher levels than the gentamicin group. Experimental groups also exhibited significantly increased Malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GSH-Px) with more pronounced effects in the cisplatin-treated group. Further, both experimental groups exhibited significant up-regulation of Tumor Necrosis Factor α (TNF-α), caspase-3, and Bax and down regulation of Bcl-2. CONCLUSION: These findings confirm the use of necrotic, apoptotic genes as early biomarkers in the detection of tubular kidney damage. Further, cisplatin was shown to have a greater nephrotoxic effect than gentamicin; therefore, its use should be constrained accordingly when co-administered with gentamicin. BioMed Central 2021-11-16 /pmc/articles/PMC8594120/ /pubmed/34784920 http://dx.doi.org/10.1186/s12917-021-03023-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Abouzed, Tarek Kamal
Sherif, Eman Abd Elrahman
Barakat, Mohamed El Sayed
Sadek, Kadry Mohamed
Aldhahrani, Adil
Nasr, Nasr Elsayed
Eldomany, Ehab
Khailo, Khaled
Dorghamm, Doaa Abdallha
Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_full Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_fullStr Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_full_unstemmed Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_short Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
title_sort assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594120/
https://www.ncbi.nlm.nih.gov/pubmed/34784920
http://dx.doi.org/10.1186/s12917-021-03023-4
work_keys_str_mv AT abouzedtarekkamal assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT sherifemanabdelrahman assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT barakatmohamedelsayed assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT sadekkadrymohamed assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT aldhahraniadil assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT nasrnasrelsayed assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT eldomanyehab assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT khailokhaled assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats
AT dorghammdoaaabdallha assessmentofgentamicinandcisplatininducedkidneydamagemediatedvianecroticandapoptosisgenesinalbinorats