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Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis
DNA methylation is an important epigenetic mark involved in gene regulation and silencing of transposable elements. The presence or absence of DNA methylation at specific sites can influence nearby gene expression and cause phenotypic changes that remain stable over generations. Recently, developmen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594676/ https://www.ncbi.nlm.nih.gov/pubmed/34968243 http://dx.doi.org/10.3390/epigenomes4020009 |
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author | Gardiner, Jason Zhao, Jenny M. Chaffin, Kendall Jacobsen, Steven E. |
author_facet | Gardiner, Jason Zhao, Jenny M. Chaffin, Kendall Jacobsen, Steven E. |
author_sort | Gardiner, Jason |
collection | PubMed |
description | DNA methylation is an important epigenetic mark involved in gene regulation and silencing of transposable elements. The presence or absence of DNA methylation at specific sites can influence nearby gene expression and cause phenotypic changes that remain stable over generations. Recently, development of new technologies has enabled the targeted addition or removal of DNA methylation at specific sites of the genome. Of these new technologies, the targeting of the catalytic domain of Nicotiana tabacum DOMAINS REARRANGED METHYLTRANSFERASE 2 (ntDRM2cd) offers a promising tool for the addition of DNA methylation as it can directly methylate DNA. However, the methylation targeting efficiency of constructs using ntDRM2cd thus far has been relatively low. Previous studies have shown that the use of different promoters or terminators can greatly improve genome-editing efficiencies. In this study, we systematically survey a variety of promoter and terminator combinations to identify optimal combinations to use when targeting the addition of DNA methylation in Arabidopsis thaliana. |
format | Online Article Text |
id | pubmed-8594676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85946762021-12-28 Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis Gardiner, Jason Zhao, Jenny M. Chaffin, Kendall Jacobsen, Steven E. Epigenomes Article DNA methylation is an important epigenetic mark involved in gene regulation and silencing of transposable elements. The presence or absence of DNA methylation at specific sites can influence nearby gene expression and cause phenotypic changes that remain stable over generations. Recently, development of new technologies has enabled the targeted addition or removal of DNA methylation at specific sites of the genome. Of these new technologies, the targeting of the catalytic domain of Nicotiana tabacum DOMAINS REARRANGED METHYLTRANSFERASE 2 (ntDRM2cd) offers a promising tool for the addition of DNA methylation as it can directly methylate DNA. However, the methylation targeting efficiency of constructs using ntDRM2cd thus far has been relatively low. Previous studies have shown that the use of different promoters or terminators can greatly improve genome-editing efficiencies. In this study, we systematically survey a variety of promoter and terminator combinations to identify optimal combinations to use when targeting the addition of DNA methylation in Arabidopsis thaliana. MDPI 2020-06-12 /pmc/articles/PMC8594676/ /pubmed/34968243 http://dx.doi.org/10.3390/epigenomes4020009 Text en © 2020 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Gardiner, Jason Zhao, Jenny M. Chaffin, Kendall Jacobsen, Steven E. Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis |
title | Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis |
title_full | Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis |
title_fullStr | Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis |
title_full_unstemmed | Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis |
title_short | Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis |
title_sort | promoter and terminator optimization for dna methylation targeting in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594676/ https://www.ncbi.nlm.nih.gov/pubmed/34968243 http://dx.doi.org/10.3390/epigenomes4020009 |
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