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Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag

Epigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription factors and histone modifications. A recently established method called CUT&Tag uses a Protein-A Tn5 transposase fusion protein, which cuts the genome and inserts adapt...

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Detalles Bibliográficos
Autores principales: Fujiwara, Yasuhiro, Tanno, Yuji, Sugishita, Hiroki, Kishi, Yusuke, Makino, Yoshinori, Okada, Yuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594809/
https://www.ncbi.nlm.nih.gov/pubmed/34784358
http://dx.doi.org/10.1371/journal.pone.0259846