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Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis

Myocardial ischemia-reperfusion injury (MIRI) is a phenomenon that reperfusion leads to irreversible damage to the myocardium and increases mortality in acute myocardial infarction (AMI) patients. There is no effective drug to treat MIRI. Tubeimoside I (TBM) is a triterpenoid saponin purified from C...

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Autores principales: Lv, Dingyi, Luo, Minghao, Cheng, Zhe, Wang, Ruiyu, Yang, Xiyang, Guo, Yongzheng, Huang, Longxiang, Li, Xiang, Huang, Bi, Shen, Jian, Luo, Suxin, Yan, Jianghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595016/
https://www.ncbi.nlm.nih.gov/pubmed/34795840
http://dx.doi.org/10.1155/2021/5577019
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author Lv, Dingyi
Luo, Minghao
Cheng, Zhe
Wang, Ruiyu
Yang, Xiyang
Guo, Yongzheng
Huang, Longxiang
Li, Xiang
Huang, Bi
Shen, Jian
Luo, Suxin
Yan, Jianghong
author_facet Lv, Dingyi
Luo, Minghao
Cheng, Zhe
Wang, Ruiyu
Yang, Xiyang
Guo, Yongzheng
Huang, Longxiang
Li, Xiang
Huang, Bi
Shen, Jian
Luo, Suxin
Yan, Jianghong
author_sort Lv, Dingyi
collection PubMed
description Myocardial ischemia-reperfusion injury (MIRI) is a phenomenon that reperfusion leads to irreversible damage to the myocardium and increases mortality in acute myocardial infarction (AMI) patients. There is no effective drug to treat MIRI. Tubeimoside I (TBM) is a triterpenoid saponin purified from Chinese traditional medicine tubeimu. In this study, 4 mg/kg TBM was given to mice intraperitoneally at 15 min after ischemia. And TBM treatment improved postischemic cardiac function, decreased infarct size, diminished lactate dehydrogenase release, ameliorated oxidative stress, and reduced apoptotic index. Notably, ischemia-reperfusion induced a significant decrease in cardiac SIRT3 expression and activity, while TBM treatment upregulated SIRT3's expression and activity. However, the cardioprotective effects of TBM were largely abolished by a SIRT3 inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP). This suggests that SIRT3 plays an essential role in TBM's cardioprotective effects. In vitro, TBM also protected H9c2 cells against simulated ischemia/reperfusion (SIR) injury by attenuating oxidative stress and apoptosis, and siSIRT3 diminished its protective effects. Taken together, our results demonstrate for the first time that TBM protects against MIRI through SIRT3-dependent regulation of oxidative stress and apoptosis. TBM might be a potential drug candidate for MIRI treatment.
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spelling pubmed-85950162021-11-17 Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis Lv, Dingyi Luo, Minghao Cheng, Zhe Wang, Ruiyu Yang, Xiyang Guo, Yongzheng Huang, Longxiang Li, Xiang Huang, Bi Shen, Jian Luo, Suxin Yan, Jianghong Oxid Med Cell Longev Research Article Myocardial ischemia-reperfusion injury (MIRI) is a phenomenon that reperfusion leads to irreversible damage to the myocardium and increases mortality in acute myocardial infarction (AMI) patients. There is no effective drug to treat MIRI. Tubeimoside I (TBM) is a triterpenoid saponin purified from Chinese traditional medicine tubeimu. In this study, 4 mg/kg TBM was given to mice intraperitoneally at 15 min after ischemia. And TBM treatment improved postischemic cardiac function, decreased infarct size, diminished lactate dehydrogenase release, ameliorated oxidative stress, and reduced apoptotic index. Notably, ischemia-reperfusion induced a significant decrease in cardiac SIRT3 expression and activity, while TBM treatment upregulated SIRT3's expression and activity. However, the cardioprotective effects of TBM were largely abolished by a SIRT3 inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP). This suggests that SIRT3 plays an essential role in TBM's cardioprotective effects. In vitro, TBM also protected H9c2 cells against simulated ischemia/reperfusion (SIR) injury by attenuating oxidative stress and apoptosis, and siSIRT3 diminished its protective effects. Taken together, our results demonstrate for the first time that TBM protects against MIRI through SIRT3-dependent regulation of oxidative stress and apoptosis. TBM might be a potential drug candidate for MIRI treatment. Hindawi 2021-11-09 /pmc/articles/PMC8595016/ /pubmed/34795840 http://dx.doi.org/10.1155/2021/5577019 Text en Copyright © 2021 Dingyi Lv et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lv, Dingyi
Luo, Minghao
Cheng, Zhe
Wang, Ruiyu
Yang, Xiyang
Guo, Yongzheng
Huang, Longxiang
Li, Xiang
Huang, Bi
Shen, Jian
Luo, Suxin
Yan, Jianghong
Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis
title Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis
title_full Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis
title_fullStr Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis
title_full_unstemmed Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis
title_short Tubeimoside I Ameliorates Myocardial Ischemia-Reperfusion Injury through SIRT3-Dependent Regulation of Oxidative Stress and Apoptosis
title_sort tubeimoside i ameliorates myocardial ischemia-reperfusion injury through sirt3-dependent regulation of oxidative stress and apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595016/
https://www.ncbi.nlm.nih.gov/pubmed/34795840
http://dx.doi.org/10.1155/2021/5577019
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