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Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs

There is a critical need to develop superior influenza vaccines that provide broader protection. Influenza vaccines are traditionally tested in naive animals, although humans are exposed to influenza in the first years of their lives, but the impact of prior influenza exposure on vaccine immune resp...

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Detalles Bibliográficos
Autores principales: Vatzia, Eleni, Allen, Elizabeth R., Manjegowda, Tanuja, Morris, Susan, McNee, Adam, Martini, Veronica, Kaliath, Reshma, Ulaszewska, Marta, Boyd, Amy, Paudyal, Basudev, Carr, Veronica B., Chrun, Tiphany, Maze, Emmanuel, MacLoughlin, Ronan, van Diemen, Pauline M., Everett, Helen E., Lambe, Teresa, Gilbert, Sarah C., Tchilian, Elma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595216/
https://www.ncbi.nlm.nih.gov/pubmed/34804053
http://dx.doi.org/10.3389/fimmu.2021.763912
Descripción
Sumario:There is a critical need to develop superior influenza vaccines that provide broader protection. Influenza vaccines are traditionally tested in naive animals, although humans are exposed to influenza in the first years of their lives, but the impact of prior influenza exposure on vaccine immune responses has not been well studied. Pigs are an important natural host for influenza, are a source of pandemic viruses, and are an excellent model for human influenza. Here, we investigated the immunogenicity of the ChAdOx2 viral vectored vaccine, expressing influenza nucleoprotein, matrix protein 1, and neuraminidase in H1N1pdm09 pre-exposed pigs. We evaluated the importance of the route of administration by comparing intranasal, aerosol, and intramuscular immunizations. Aerosol delivery boosted the local lung T-cell and antibody responses, while intramuscular immunization boosted peripheral blood immunity. These results will inform how best to deliver vaccines in order to harness optimal protective immunity.