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Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution
The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample ch...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595732/ https://www.ncbi.nlm.nih.gov/pubmed/34785666 http://dx.doi.org/10.1038/s41467-021-26837-0 |
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author | Williams, Gareth O. S. Williams, Elvira Finlayson, Neil Erdogan, Ahmet T. Wang, Qiang Fernandes, Susan Akram, Ahsan R. Dhaliwal, Kev Henderson, Robert K. Girkin, John M. Bradley, Mark |
author_facet | Williams, Gareth O. S. Williams, Elvira Finlayson, Neil Erdogan, Ahmet T. Wang, Qiang Fernandes, Susan Akram, Ahsan R. Dhaliwal, Kev Henderson, Robert K. Girkin, John M. Bradley, Mark |
author_sort | Williams, Gareth O. S. |
collection | PubMed |
description | The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems. |
format | Online Article Text |
id | pubmed-8595732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85957322021-11-19 Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution Williams, Gareth O. S. Williams, Elvira Finlayson, Neil Erdogan, Ahmet T. Wang, Qiang Fernandes, Susan Akram, Ahsan R. Dhaliwal, Kev Henderson, Robert K. Girkin, John M. Bradley, Mark Nat Commun Article The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems. Nature Publishing Group UK 2021-11-16 /pmc/articles/PMC8595732/ /pubmed/34785666 http://dx.doi.org/10.1038/s41467-021-26837-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Williams, Gareth O. S. Williams, Elvira Finlayson, Neil Erdogan, Ahmet T. Wang, Qiang Fernandes, Susan Akram, Ahsan R. Dhaliwal, Kev Henderson, Robert K. Girkin, John M. Bradley, Mark Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
title | Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
title_full | Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
title_fullStr | Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
title_full_unstemmed | Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
title_short | Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
title_sort | full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595732/ https://www.ncbi.nlm.nih.gov/pubmed/34785666 http://dx.doi.org/10.1038/s41467-021-26837-0 |
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