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Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease

Non-alcoholic fatty liver disease (NAFLD) is considered one of the most serious public health problems affecting liver. The reported beneficial impact of raspberries on obesity and associated metabolic disorder makes it a suitable candidate against NAFLD. In the current study, the chemical profile o...

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Autores principales: Hendawy, Omnia, Gomaa, Hesham A.M., Hussein, Shaimaa, Alzarea, Sami I., Qasim, Sumera, Abdel Rahman, Fatema El-Zahraa S., Ali, Asmaa T., Ahmed, Shaimaa R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596288/
https://www.ncbi.nlm.nih.gov/pubmed/34819792
http://dx.doi.org/10.1016/j.jsps.2021.09.014
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author Hendawy, Omnia
Gomaa, Hesham A.M.
Hussein, Shaimaa
Alzarea, Sami I.
Qasim, Sumera
Abdel Rahman, Fatema El-Zahraa S.
Ali, Asmaa T.
Ahmed, Shaimaa R.
author_facet Hendawy, Omnia
Gomaa, Hesham A.M.
Hussein, Shaimaa
Alzarea, Sami I.
Qasim, Sumera
Abdel Rahman, Fatema El-Zahraa S.
Ali, Asmaa T.
Ahmed, Shaimaa R.
author_sort Hendawy, Omnia
collection PubMed
description Non-alcoholic fatty liver disease (NAFLD) is considered one of the most serious public health problems affecting liver. The reported beneficial impact of raspberries on obesity and associated metabolic disorder makes it a suitable candidate against NAFLD. In the current study, the chemical profile of raspberry seed oil (RO) was characterized by analysis of fatty acid and tocopherol contents using high-performance liquid chromatography (HPLC) in addition to the determination of total phenolic and flavonoids. High levels of unsaturated fatty acids, linoleic acid (49.9%), α-linolenic acid (25.98%), and oleic acid (17.6%), along with high total tocopherol content (184 mg/100 gm) were detected in oil. The total phenolic and flavonoid contents in RO were estimated to be 22.40 ± 0.25 mg gallic acid equivalent (GAE)/100 mg oil and 1.34 ± 0.15 mg quercetin (QU)/100 mg, respectively. Anti-NAFLD efficacy of RO at different doses (0.4 and 0.8 mL) in a model of a high-fat diet (HFD) fed rats was assessed by estimating lipid profile, liver enzyme activity, glucose and insulin levels as well as adipokines and inflammatory marker. Peroxisome proliferator-activated receptor γ (PPARγ), which is a molecular target for NAFLD was also tested. Liver histopathology was carried out and its homogenate was used to estimate oxidative stress markers. Consumption of RO significantly improved lipid parameters and hepatic enzyme activities, reduced insulin resistance and glucose levels, significantly ameliorated inflammatory and oxidative stress markers. Furthermore, RO treatment significantly modulated adipokines activities and elevated PPARγ levels. Raspberry seed oil administration significantly improved these HFD induced histopathological alterations. Moreover, a molecular docking study was performed on the identified fatty acids and tocopherols. Among the identified compounds, oleic acid, α-linolenic acid and γ-tocopherol exhibited the highest docking score as PPARγ activator posing them as a potential anti-NAFLD drug leads. Study findings suggest RO as an effective therapeutic candidate for ameliorating NAFLD.
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spelling pubmed-85962882021-11-23 Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease Hendawy, Omnia Gomaa, Hesham A.M. Hussein, Shaimaa Alzarea, Sami I. Qasim, Sumera Abdel Rahman, Fatema El-Zahraa S. Ali, Asmaa T. Ahmed, Shaimaa R. Saudi Pharm J Original Article Non-alcoholic fatty liver disease (NAFLD) is considered one of the most serious public health problems affecting liver. The reported beneficial impact of raspberries on obesity and associated metabolic disorder makes it a suitable candidate against NAFLD. In the current study, the chemical profile of raspberry seed oil (RO) was characterized by analysis of fatty acid and tocopherol contents using high-performance liquid chromatography (HPLC) in addition to the determination of total phenolic and flavonoids. High levels of unsaturated fatty acids, linoleic acid (49.9%), α-linolenic acid (25.98%), and oleic acid (17.6%), along with high total tocopherol content (184 mg/100 gm) were detected in oil. The total phenolic and flavonoid contents in RO were estimated to be 22.40 ± 0.25 mg gallic acid equivalent (GAE)/100 mg oil and 1.34 ± 0.15 mg quercetin (QU)/100 mg, respectively. Anti-NAFLD efficacy of RO at different doses (0.4 and 0.8 mL) in a model of a high-fat diet (HFD) fed rats was assessed by estimating lipid profile, liver enzyme activity, glucose and insulin levels as well as adipokines and inflammatory marker. Peroxisome proliferator-activated receptor γ (PPARγ), which is a molecular target for NAFLD was also tested. Liver histopathology was carried out and its homogenate was used to estimate oxidative stress markers. Consumption of RO significantly improved lipid parameters and hepatic enzyme activities, reduced insulin resistance and glucose levels, significantly ameliorated inflammatory and oxidative stress markers. Furthermore, RO treatment significantly modulated adipokines activities and elevated PPARγ levels. Raspberry seed oil administration significantly improved these HFD induced histopathological alterations. Moreover, a molecular docking study was performed on the identified fatty acids and tocopherols. Among the identified compounds, oleic acid, α-linolenic acid and γ-tocopherol exhibited the highest docking score as PPARγ activator posing them as a potential anti-NAFLD drug leads. Study findings suggest RO as an effective therapeutic candidate for ameliorating NAFLD. Elsevier 2021-11 2021-10-06 /pmc/articles/PMC8596288/ /pubmed/34819792 http://dx.doi.org/10.1016/j.jsps.2021.09.014 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Hendawy, Omnia
Gomaa, Hesham A.M.
Hussein, Shaimaa
Alzarea, Sami I.
Qasim, Sumera
Abdel Rahman, Fatema El-Zahraa S.
Ali, Asmaa T.
Ahmed, Shaimaa R.
Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
title Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
title_full Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
title_fullStr Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
title_full_unstemmed Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
title_short Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
title_sort cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596288/
https://www.ncbi.nlm.nih.gov/pubmed/34819792
http://dx.doi.org/10.1016/j.jsps.2021.09.014
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