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Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains
Microscale fermentation systems are important high throughput tools in clone selection, and bioprocess set up and optimization, since they provide several parallel experiments in controlled conditions of pH, temperature, agitation, and gas flow rate. In this work we evaluated the performance of biot...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596446/ https://www.ncbi.nlm.nih.gov/pubmed/34180150 http://dx.doi.org/10.1002/btpr.3184 |
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author | D'ambrosio, Sergio Ventrone, Michela Alfano, Alberto Schiraldi, Chiara Cimini, Donatella |
author_facet | D'ambrosio, Sergio Ventrone, Michela Alfano, Alberto Schiraldi, Chiara Cimini, Donatella |
author_sort | D'ambrosio, Sergio |
collection | PubMed |
description | Microscale fermentation systems are important high throughput tools in clone selection, and bioprocess set up and optimization, since they provide several parallel experiments in controlled conditions of pH, temperature, agitation, and gas flow rate. In this work we evaluated the performance of biotechnologically relevant strains with different respiratory requirements in the micro‐Matrix microbioreactor. In particular Escherichia coli K4 requires well aerated fermentation conditions to improve its native production of chondroitin‐like capsular polysaccharide, a biomedically attractive polymer. Results from batch and fed‐batch experiments demonstrated high reproducibility with those obtained on 2 L reactors, although highlighting a pronounced volume loss for longer‐term experiments. Basfia succiniciproducens and Actinobacillus succinogenes need CO(2) addition for the production of succinic acid, a building block with several industrial applications. Different CO(2) supply modes were tested for the two strains in 24 h batch experiments and results well compared with those obtained on lab‐scale bioreactors. Overall, it was demonstrated that the micro‐Matrix is a useful scale‐down tool that is suitable for growing metabolically different strains in simple batch process, however, a series of issues should still be addressed in order to fully exploit its potential. |
format | Online Article Text |
id | pubmed-8596446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85964462021-11-22 Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains D'ambrosio, Sergio Ventrone, Michela Alfano, Alberto Schiraldi, Chiara Cimini, Donatella Biotechnol Prog NOTES Microscale fermentation systems are important high throughput tools in clone selection, and bioprocess set up and optimization, since they provide several parallel experiments in controlled conditions of pH, temperature, agitation, and gas flow rate. In this work we evaluated the performance of biotechnologically relevant strains with different respiratory requirements in the micro‐Matrix microbioreactor. In particular Escherichia coli K4 requires well aerated fermentation conditions to improve its native production of chondroitin‐like capsular polysaccharide, a biomedically attractive polymer. Results from batch and fed‐batch experiments demonstrated high reproducibility with those obtained on 2 L reactors, although highlighting a pronounced volume loss for longer‐term experiments. Basfia succiniciproducens and Actinobacillus succinogenes need CO(2) addition for the production of succinic acid, a building block with several industrial applications. Different CO(2) supply modes were tested for the two strains in 24 h batch experiments and results well compared with those obtained on lab‐scale bioreactors. Overall, it was demonstrated that the micro‐Matrix is a useful scale‐down tool that is suitable for growing metabolically different strains in simple batch process, however, a series of issues should still be addressed in order to fully exploit its potential. John Wiley & Sons, Inc. 2021-07-05 2021 /pmc/articles/PMC8596446/ /pubmed/34180150 http://dx.doi.org/10.1002/btpr.3184 Text en © 2021 The Authors. Biotechnology Progress published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | NOTES D'ambrosio, Sergio Ventrone, Michela Alfano, Alberto Schiraldi, Chiara Cimini, Donatella Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
title | Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
title_full | Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
title_fullStr | Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
title_full_unstemmed | Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
title_short | Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
title_sort | microbioreactor (micro‐matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains |
topic | NOTES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596446/ https://www.ncbi.nlm.nih.gov/pubmed/34180150 http://dx.doi.org/10.1002/btpr.3184 |
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