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N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions
Alpha-1,6-mannosyl-glycoprotein 2-β-N-acetylglucosaminyltransferase [EC 2.4.1.143, N-acetylglucosaminyltransferase II (GnTII)] catalyzes the transfer of N-acetylglucosamine (GlcNAc) residue from the nucleotide sugar donor UDP-GlcNAc to the α1,6-mannose residue of the di-antennary N-glycan acceptor G...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596550/ https://www.ncbi.nlm.nih.gov/pubmed/34804097 http://dx.doi.org/10.3389/fpls.2021.761064 |
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author | Yoo, Jae Yong Ko, Ki Seong Vu, Bich Ngoc Lee, Young Eun Yoon, Seok Han Pham, Thao Thi Kim, Ji-Yeon Lim, Jae-Min Kang, Yang Jae Hong, Jong Chan Lee, Kyun Oh |
author_facet | Yoo, Jae Yong Ko, Ki Seong Vu, Bich Ngoc Lee, Young Eun Yoon, Seok Han Pham, Thao Thi Kim, Ji-Yeon Lim, Jae-Min Kang, Yang Jae Hong, Jong Chan Lee, Kyun Oh |
author_sort | Yoo, Jae Yong |
collection | PubMed |
description | Alpha-1,6-mannosyl-glycoprotein 2-β-N-acetylglucosaminyltransferase [EC 2.4.1.143, N-acetylglucosaminyltransferase II (GnTII)] catalyzes the transfer of N-acetylglucosamine (GlcNAc) residue from the nucleotide sugar donor UDP-GlcNAc to the α1,6-mannose residue of the di-antennary N-glycan acceptor GlcNAc(Xyl)Man(3)(Fuc)GlcNAc(2) in the Golgi apparatus. Although the formation of the GlcNAc(2)(Xyl)Man(3)(Fuc)GlcNAc(2) N-glycan is known to be associated with GnTII activity in Arabidopsis thaliana, its physiological significance is still not fully understood in plants. To address the physiological importance of the GlcNAc(2)(Xyl)Man(3)(Fuc)GlcNAc(2) N-glycan, we examined the phenotypic effects of loss-of-function mutations in GnTII in the presence and absence of stress, and responsiveness to phytohormones. Prolonged stress induced by tunicamycin (TM) or sodium chloride (NaCl) treatment increased GnTII expression in wild-type Arabidopsis (ecotype Col-0) but caused severe developmental damage in GnTII loss-of-function mutants (gnt2-1 and gnt2-2). The absence of the 6-arm GlcNAc residue in the N-glycans in gnt2-1 facilitated the TM-induced unfolded protein response, accelerated dark-induced leaf senescence, and reduced cytokinin signaling, as well as susceptibility to cytokinin-induced root growth inhibition. Furthermore, gnt2-1 and gnt2-2 seedlings exhibited enhanced N-1-naphthylphthalamic acid-induced inhibition of tropic growth and development. Thus, GnTII’s promotion of the 6-arm GlcNAc addition to N-glycans is important for plant growth and development under stress conditions, possibly via affecting glycoprotein folding and/or distribution. |
format | Online Article Text |
id | pubmed-8596550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85965502021-11-18 N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions Yoo, Jae Yong Ko, Ki Seong Vu, Bich Ngoc Lee, Young Eun Yoon, Seok Han Pham, Thao Thi Kim, Ji-Yeon Lim, Jae-Min Kang, Yang Jae Hong, Jong Chan Lee, Kyun Oh Front Plant Sci Plant Science Alpha-1,6-mannosyl-glycoprotein 2-β-N-acetylglucosaminyltransferase [EC 2.4.1.143, N-acetylglucosaminyltransferase II (GnTII)] catalyzes the transfer of N-acetylglucosamine (GlcNAc) residue from the nucleotide sugar donor UDP-GlcNAc to the α1,6-mannose residue of the di-antennary N-glycan acceptor GlcNAc(Xyl)Man(3)(Fuc)GlcNAc(2) in the Golgi apparatus. Although the formation of the GlcNAc(2)(Xyl)Man(3)(Fuc)GlcNAc(2) N-glycan is known to be associated with GnTII activity in Arabidopsis thaliana, its physiological significance is still not fully understood in plants. To address the physiological importance of the GlcNAc(2)(Xyl)Man(3)(Fuc)GlcNAc(2) N-glycan, we examined the phenotypic effects of loss-of-function mutations in GnTII in the presence and absence of stress, and responsiveness to phytohormones. Prolonged stress induced by tunicamycin (TM) or sodium chloride (NaCl) treatment increased GnTII expression in wild-type Arabidopsis (ecotype Col-0) but caused severe developmental damage in GnTII loss-of-function mutants (gnt2-1 and gnt2-2). The absence of the 6-arm GlcNAc residue in the N-glycans in gnt2-1 facilitated the TM-induced unfolded protein response, accelerated dark-induced leaf senescence, and reduced cytokinin signaling, as well as susceptibility to cytokinin-induced root growth inhibition. Furthermore, gnt2-1 and gnt2-2 seedlings exhibited enhanced N-1-naphthylphthalamic acid-induced inhibition of tropic growth and development. Thus, GnTII’s promotion of the 6-arm GlcNAc addition to N-glycans is important for plant growth and development under stress conditions, possibly via affecting glycoprotein folding and/or distribution. Frontiers Media S.A. 2021-11-02 /pmc/articles/PMC8596550/ /pubmed/34804097 http://dx.doi.org/10.3389/fpls.2021.761064 Text en Copyright © 2021 Yoo, Ko, Vu, Lee, Yoon, Pham, Kim, Lim, Kang, Hong and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yoo, Jae Yong Ko, Ki Seong Vu, Bich Ngoc Lee, Young Eun Yoon, Seok Han Pham, Thao Thi Kim, Ji-Yeon Lim, Jae-Min Kang, Yang Jae Hong, Jong Chan Lee, Kyun Oh N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions |
title | N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions |
title_full | N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions |
title_fullStr | N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions |
title_full_unstemmed | N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions |
title_short | N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditions |
title_sort | n-acetylglucosaminyltransferase ii is involved in plant growth and development under stress conditions |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596550/ https://www.ncbi.nlm.nih.gov/pubmed/34804097 http://dx.doi.org/10.3389/fpls.2021.761064 |
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