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Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS

The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatography-high resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis inducti...

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Autores principales: YAZICI BEKTAŞ, Nurdan, ERSOY, Ezgi, BOĞA, Mehmet, BORAN, Tuğçe, ÇINAR, Ercan, ÖZHAN, Gül, GÖREN, Ahmet Ceyhan, EROĞLU ÖZKAN, Esra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596555/
https://www.ncbi.nlm.nih.gov/pubmed/34849072
http://dx.doi.org/10.3906/kim-2104-17
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author YAZICI BEKTAŞ, Nurdan
ERSOY, Ezgi
BOĞA, Mehmet
BORAN, Tuğçe
ÇINAR, Ercan
ÖZHAN, Gül
GÖREN, Ahmet Ceyhan
EROĞLU ÖZKAN, Esra
author_facet YAZICI BEKTAŞ, Nurdan
ERSOY, Ezgi
BOĞA, Mehmet
BORAN, Tuğçe
ÇINAR, Ercan
ÖZHAN, Gül
GÖREN, Ahmet Ceyhan
EROĞLU ÖZKAN, Esra
author_sort YAZICI BEKTAŞ, Nurdan
collection PubMed
description The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatography-high resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 ± 70.10 μg/g) in the leaves dichloromethane (LD), herniarin (167 ± 1.50 μg/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 ± 235.36 μg/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 ± 20.46 μg/g) in the fruit methanol (FM), and caffeic acid (370 ± 8.88 μg/g) in the fruit water (FW), and (3313 ± 79.51 μg/g) in the leaves water (LW) extracts. LM exerted strong antioxidant activity in DPPH free (IC(50) 10.94 ± 0.08 μg/mL), and ABTS cation radicals scavenging (IC(50) 9.09 ± 0.05 μg/mL) activities. FM exhibited cytotoxic activity with IC(50 )values of 73.23 ± 3.06 µg/mL and 31.64 ± 2.75 µg/mL on PC-3 and Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 ± 18.9 μg/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction ability on different cells, FM extract showed apoptosis induction activity with 64.75% on PC-3 cells at 50 μg/mL concentration. The study provides promising results about the potential of Hypericum androsaemum on cancer prevention.
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spelling pubmed-85965552021-11-29 Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS YAZICI BEKTAŞ, Nurdan ERSOY, Ezgi BOĞA, Mehmet BORAN, Tuğçe ÇINAR, Ercan ÖZHAN, Gül GÖREN, Ahmet Ceyhan EROĞLU ÖZKAN, Esra Turk J Chem Article The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatography-high resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 ± 70.10 μg/g) in the leaves dichloromethane (LD), herniarin (167 ± 1.50 μg/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 ± 235.36 μg/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 ± 20.46 μg/g) in the fruit methanol (FM), and caffeic acid (370 ± 8.88 μg/g) in the fruit water (FW), and (3313 ± 79.51 μg/g) in the leaves water (LW) extracts. LM exerted strong antioxidant activity in DPPH free (IC(50) 10.94 ± 0.08 μg/mL), and ABTS cation radicals scavenging (IC(50) 9.09 ± 0.05 μg/mL) activities. FM exhibited cytotoxic activity with IC(50 )values of 73.23 ± 3.06 µg/mL and 31.64 ± 2.75 µg/mL on PC-3 and Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 ± 18.9 μg/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction ability on different cells, FM extract showed apoptosis induction activity with 64.75% on PC-3 cells at 50 μg/mL concentration. The study provides promising results about the potential of Hypericum androsaemum on cancer prevention. The Scientific and Technological Research Council of Turkey 2021-10-19 /pmc/articles/PMC8596555/ /pubmed/34849072 http://dx.doi.org/10.3906/kim-2104-17 Text en Copyright © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
YAZICI BEKTAŞ, Nurdan
ERSOY, Ezgi
BOĞA, Mehmet
BORAN, Tuğçe
ÇINAR, Ercan
ÖZHAN, Gül
GÖREN, Ahmet Ceyhan
EROĞLU ÖZKAN, Esra
Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
title Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
title_full Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
title_fullStr Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
title_full_unstemmed Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
title_short Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
title_sort cytotoxic and apoptotic effects of hypericum androsaemum on prostate adenocarcinoma (pc-3) and hepatocellular carcinoma (hep g2) cell lines with identification of secondary metabolites by lc-hrms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596555/
https://www.ncbi.nlm.nih.gov/pubmed/34849072
http://dx.doi.org/10.3906/kim-2104-17
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