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Controlling Gene Expression in Mammalian Cells Using Multiplexed Conditional Guide RNAs for Cas12a

Spatiotemporal control of the activity of CRISPR‐associated (Cas) proteins is of considerable interest for basic research and therapeutics. Here, we show that conditional guide RNAs (gRNAs) for Cas12a can be transcribed in mammalian cells by RNA polymerase II, followed by activation via input‐depend...

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Detalles Bibliográficos
Autores principales:	Oesinghaus, Lukas, Simmel, Friedrich C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596743/ https://www.ncbi.nlm.nih.gov/pubmed/34533878 http://dx.doi.org/10.1002/anie.202107258

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