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Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation

The South American tomato pinworm, Tuta absoluta, is one of the most destructive insect pests in Solanaceae crops, particularly in tomatoes. Current methods of management have proven somewhat effective but still require a more efficacious management strategy to limit its havoc on crop yield. Tomato...

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Autores principales: Chen, Limin, Li, Xiaowei, Zhang, Jinming, He, Tianjun, Huang, Jun, Zhang, Zhijun, Wang, Yeyang, Hafeez, Muhammad, Zhou, Shuxing, Ren, Xiaoyun, Hou, Youming, Lu, Yaobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597266/
https://www.ncbi.nlm.nih.gov/pubmed/34804095
http://dx.doi.org/10.3389/fpls.2021.757230
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author Chen, Limin
Li, Xiaowei
Zhang, Jinming
He, Tianjun
Huang, Jun
Zhang, Zhijun
Wang, Yeyang
Hafeez, Muhammad
Zhou, Shuxing
Ren, Xiaoyun
Hou, Youming
Lu, Yaobin
author_facet Chen, Limin
Li, Xiaowei
Zhang, Jinming
He, Tianjun
Huang, Jun
Zhang, Zhijun
Wang, Yeyang
Hafeez, Muhammad
Zhou, Shuxing
Ren, Xiaoyun
Hou, Youming
Lu, Yaobin
author_sort Chen, Limin
collection PubMed
description The South American tomato pinworm, Tuta absoluta, is one of the most destructive insect pests in Solanaceae crops, particularly in tomatoes. Current methods of management have proven somewhat effective but still require a more efficacious management strategy to limit its havoc on crop yield. Tomato is much more predisposed to T. absoluta as compared with other plants such as eggplants, but the underlying causes have not been fully determined. We conducted this study to unravel the volatile organic compounds (VOCs) and primary/secondary metabolites that account for the differential response of tomatoes and eggplants to T. absoluta infestation. We performed widely targeted comparative metabolome and volatilome profiling by ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) and headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC-MS), respectively, on eggplants and tomatoes under control and T. absoluta infestation conditions. Overall, 141 VOCs and 797 primary/secondary metabolites were identified, largely dominated by aldehyde, alcohols, alkanes, amine, aromatics, a heterocyclic compound, ketone, olefin, phenol, and terpenes. Most of the VOCs and primary/secondary metabolites from the terpene class were largely differentially regulated in eggplants compared with tomatoes. Eggplants emitted several compounds that were lower or completely absent in tomatoes either under control conditions or after T. absoluta infestation. The results from an electroantennogram showed that 35 differentially accumulated VOCs could elicit female T. absoluta response, implying that these volatile compounds significantly alter the behavior of this pest. These findings demonstrated that differentially accumulated metabolites and volatile compounds play major roles in eggplant resistance to T. absoluta infestation as these compounds were regulated upon attack by T. absoluta. Our findings can assist in integrated pest management efforts by developing appropriate control measures against T. absoluta in Solanaceae production.
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spelling pubmed-85972662021-11-18 Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation Chen, Limin Li, Xiaowei Zhang, Jinming He, Tianjun Huang, Jun Zhang, Zhijun Wang, Yeyang Hafeez, Muhammad Zhou, Shuxing Ren, Xiaoyun Hou, Youming Lu, Yaobin Front Plant Sci Plant Science The South American tomato pinworm, Tuta absoluta, is one of the most destructive insect pests in Solanaceae crops, particularly in tomatoes. Current methods of management have proven somewhat effective but still require a more efficacious management strategy to limit its havoc on crop yield. Tomato is much more predisposed to T. absoluta as compared with other plants such as eggplants, but the underlying causes have not been fully determined. We conducted this study to unravel the volatile organic compounds (VOCs) and primary/secondary metabolites that account for the differential response of tomatoes and eggplants to T. absoluta infestation. We performed widely targeted comparative metabolome and volatilome profiling by ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) and headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC-MS), respectively, on eggplants and tomatoes under control and T. absoluta infestation conditions. Overall, 141 VOCs and 797 primary/secondary metabolites were identified, largely dominated by aldehyde, alcohols, alkanes, amine, aromatics, a heterocyclic compound, ketone, olefin, phenol, and terpenes. Most of the VOCs and primary/secondary metabolites from the terpene class were largely differentially regulated in eggplants compared with tomatoes. Eggplants emitted several compounds that were lower or completely absent in tomatoes either under control conditions or after T. absoluta infestation. The results from an electroantennogram showed that 35 differentially accumulated VOCs could elicit female T. absoluta response, implying that these volatile compounds significantly alter the behavior of this pest. These findings demonstrated that differentially accumulated metabolites and volatile compounds play major roles in eggplant resistance to T. absoluta infestation as these compounds were regulated upon attack by T. absoluta. Our findings can assist in integrated pest management efforts by developing appropriate control measures against T. absoluta in Solanaceae production. Frontiers Media S.A. 2021-11-03 /pmc/articles/PMC8597266/ /pubmed/34804095 http://dx.doi.org/10.3389/fpls.2021.757230 Text en Copyright © 2021 Chen, Li, Zhang, He, Huang, Zhang, Wang, Hafeez, Zhou, Ren, Hou and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chen, Limin
Li, Xiaowei
Zhang, Jinming
He, Tianjun
Huang, Jun
Zhang, Zhijun
Wang, Yeyang
Hafeez, Muhammad
Zhou, Shuxing
Ren, Xiaoyun
Hou, Youming
Lu, Yaobin
Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation
title Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation
title_full Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation
title_fullStr Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation
title_full_unstemmed Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation
title_short Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation
title_sort comprehensive metabolome and volatilome analyses in eggplant and tomato reveal their differential responses to tuta absoluta infestation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597266/
https://www.ncbi.nlm.nih.gov/pubmed/34804095
http://dx.doi.org/10.3389/fpls.2021.757230
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