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Assessment of the aortic wall histological changes with ageing
Aim: The authors aimed to quantitatively assess the variation with age of three of the main components of the aortic wall, namely elastic fibers (FE) and collagen fibers [FC(COL)], and smooth muscle cells (SMCs). Materials and Methods: Four aortic cross sections (base, cross, thoracic, abdominal) we...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597368/ https://www.ncbi.nlm.nih.gov/pubmed/34609411 http://dx.doi.org/10.47162/RJME.62.1.08 |
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author | Albu, Mirela Şeicaru, Doru Adrian Pleşea, Răzvan Mihail Mirea, Oana Cristina Gherghiceanu, Florentina Grigorean, Valentin Titus Cordoş, Ioan Liţescu, Mircea Pleşea, Iancu Emil Şerbănescu, Mircea-Sebastian |
author_facet | Albu, Mirela Şeicaru, Doru Adrian Pleşea, Răzvan Mihail Mirea, Oana Cristina Gherghiceanu, Florentina Grigorean, Valentin Titus Cordoş, Ioan Liţescu, Mircea Pleşea, Iancu Emil Şerbănescu, Mircea-Sebastian |
author_sort | Albu, Mirela |
collection | PubMed |
description | Aim: The authors aimed to quantitatively assess the variation with age of three of the main components of the aortic wall, namely elastic fibers (FE) and collagen fibers [FC(COL)], and smooth muscle cells (SMCs). Materials and Methods: Four aortic cross sections (base, cross, thoracic, abdominal) were collected during autopsy from 90 cases of all ages, processed using the classical histopathological (HP) technique (formalin fixation and paraffin embedding) and stained with Orcein and Goldner’s trichrome. The obtained histological slides were transformed into virtual slides. Quantitative measurements of the three components were made on identical regions of interest (ROIs) selected from two-paired slides stained with the above-mentioned techniques using custom-made software, developed in MATLAB (MathWorks, USA). Results and Discussions: FE revealed an obvious decreasing general trend with age, present at all four levels of investigation. Smooth muscle fibers (FM) density showed almost no variation with age regardless of the level at which the measurement was made. FC(COL) density had an obvious increasing trend with age, expressed in all four aortic regions FE densities and FM densities were higher in men than in women, while FC(COL) densities were higher in women than in men in three of the aortic regions excepting, in all cases, the cross region. Conclusions: There were differences between men and women concerning the composition of aortic wall cellular and extracellular compartments. FE and FC(COL) dominated the age-related remodeling process of the aortic wall. The process evolved in the same way in all regions of the aorta. Studies need to be continued to define more clearly this complex process of vascular wall remodeling with aging. |
format | Online Article Text |
id | pubmed-8597368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest |
record_format | MEDLINE/PubMed |
spelling | pubmed-85973682021-12-01 Assessment of the aortic wall histological changes with ageing Albu, Mirela Şeicaru, Doru Adrian Pleşea, Răzvan Mihail Mirea, Oana Cristina Gherghiceanu, Florentina Grigorean, Valentin Titus Cordoş, Ioan Liţescu, Mircea Pleşea, Iancu Emil Şerbănescu, Mircea-Sebastian Rom J Morphol Embryol Original Paper Aim: The authors aimed to quantitatively assess the variation with age of three of the main components of the aortic wall, namely elastic fibers (FE) and collagen fibers [FC(COL)], and smooth muscle cells (SMCs). Materials and Methods: Four aortic cross sections (base, cross, thoracic, abdominal) were collected during autopsy from 90 cases of all ages, processed using the classical histopathological (HP) technique (formalin fixation and paraffin embedding) and stained with Orcein and Goldner’s trichrome. The obtained histological slides were transformed into virtual slides. Quantitative measurements of the three components were made on identical regions of interest (ROIs) selected from two-paired slides stained with the above-mentioned techniques using custom-made software, developed in MATLAB (MathWorks, USA). Results and Discussions: FE revealed an obvious decreasing general trend with age, present at all four levels of investigation. Smooth muscle fibers (FM) density showed almost no variation with age regardless of the level at which the measurement was made. FC(COL) density had an obvious increasing trend with age, expressed in all four aortic regions FE densities and FM densities were higher in men than in women, while FC(COL) densities were higher in women than in men in three of the aortic regions excepting, in all cases, the cross region. Conclusions: There were differences between men and women concerning the composition of aortic wall cellular and extracellular compartments. FE and FC(COL) dominated the age-related remodeling process of the aortic wall. The process evolved in the same way in all regions of the aorta. Studies need to be continued to define more clearly this complex process of vascular wall remodeling with aging. Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest 2021 2021-08-19 /pmc/articles/PMC8597368/ /pubmed/34609411 http://dx.doi.org/10.47162/RJME.62.1.08 Text en Copyright © 2020, Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited. |
spellingShingle | Original Paper Albu, Mirela Şeicaru, Doru Adrian Pleşea, Răzvan Mihail Mirea, Oana Cristina Gherghiceanu, Florentina Grigorean, Valentin Titus Cordoş, Ioan Liţescu, Mircea Pleşea, Iancu Emil Şerbănescu, Mircea-Sebastian Assessment of the aortic wall histological changes with ageing |
title | Assessment of the aortic wall histological changes with ageing |
title_full | Assessment of the aortic wall histological changes with ageing |
title_fullStr | Assessment of the aortic wall histological changes with ageing |
title_full_unstemmed | Assessment of the aortic wall histological changes with ageing |
title_short | Assessment of the aortic wall histological changes with ageing |
title_sort | assessment of the aortic wall histological changes with ageing |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597368/ https://www.ncbi.nlm.nih.gov/pubmed/34609411 http://dx.doi.org/10.47162/RJME.62.1.08 |
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