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Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study
Skin burns are one of the most common injuries associated with increased morbidity and mortality, especially in the children and the elderlies. Severe burns, especially, result in a systemic immune and inflammatory response, which may reflect in multiple organ insufficiency, and a fast and effective...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597381/ https://www.ncbi.nlm.nih.gov/pubmed/34609418 http://dx.doi.org/10.47162/RJME.62.1.15 |
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author | Liliac, Ilona Mihaela Popescu, Elena Leocadia Văduva, Ion Alexandru Pirici, Daniel Mogoşanu, George Dan Streba, Costin Teodor Busuioc, Cristina Jana Bejenaru, Ludovic Everard Bejenaru, Cornelia Crăciunoiu, Nicolae Dumitru, Ilie Elayan, Hazim Mogoantă, Laurenţiu |
author_facet | Liliac, Ilona Mihaela Popescu, Elena Leocadia Văduva, Ion Alexandru Pirici, Daniel Mogoşanu, George Dan Streba, Costin Teodor Busuioc, Cristina Jana Bejenaru, Ludovic Everard Bejenaru, Cornelia Crăciunoiu, Nicolae Dumitru, Ilie Elayan, Hazim Mogoantă, Laurenţiu |
author_sort | Liliac, Ilona Mihaela |
collection | PubMed |
description | Skin burns are one of the most common injuries associated with increased morbidity and mortality, especially in the children and the elderlies. Severe burns, especially, result in a systemic immune and inflammatory response, which may reflect in multiple organ insufficiency, and a fast and effective local restorative process is essential for functionality recovering, as well as for interrupting the generalized systemic response. We have aimed here to assess the effect of different wound dressings in what it regards the morphology and clinical restoration after a skin burn. On a rat animal model, we have evaluated the macroscopic and histopathological features of controlled third degree skin burns in control animals versus treatments with local dressings of silver sulfadiazine (SDA) cream, simple gel (G), gel + silver nanoparticles (AgNPs) (G+NPS), gel + exosomes (G+EXO) and gel + AgNPs + exosomes (Gel+NPS+EXO), at 14 days and, respectively, 21 days after the lesion. Tissue fragments were harvested and processed for histopathology and immunohistochemistry. Immunofluorescence was utilized to evaluate the maturity of underlaying granulation tissue based on double stainings for smooth muscle actin (SMA) and cluster of differentiation 31 (CD31). Our study showed variability in what it regards the vessel density and immunoexpression of SMA between the treatments, and image analysis revealed that most SMA reduction and blood vessel density reduction in the maturing granulation tissue occurred for the G+NPS and G+NPS+EXO treatments. A complete re-epithelization was also observed for the G+NPS+EXO treatment. Overall, our results show that improved topic treatments promote faster re-epithelization and reparation of the dermis after skin burn lesions, providing thus an avenue for new treatments that aim both local recuperation and systemic infection prevention. |
format | Online Article Text |
id | pubmed-8597381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest |
record_format | MEDLINE/PubMed |
spelling | pubmed-85973812021-12-01 Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study Liliac, Ilona Mihaela Popescu, Elena Leocadia Văduva, Ion Alexandru Pirici, Daniel Mogoşanu, George Dan Streba, Costin Teodor Busuioc, Cristina Jana Bejenaru, Ludovic Everard Bejenaru, Cornelia Crăciunoiu, Nicolae Dumitru, Ilie Elayan, Hazim Mogoantă, Laurenţiu Rom J Morphol Embryol Original Paper Skin burns are one of the most common injuries associated with increased morbidity and mortality, especially in the children and the elderlies. Severe burns, especially, result in a systemic immune and inflammatory response, which may reflect in multiple organ insufficiency, and a fast and effective local restorative process is essential for functionality recovering, as well as for interrupting the generalized systemic response. We have aimed here to assess the effect of different wound dressings in what it regards the morphology and clinical restoration after a skin burn. On a rat animal model, we have evaluated the macroscopic and histopathological features of controlled third degree skin burns in control animals versus treatments with local dressings of silver sulfadiazine (SDA) cream, simple gel (G), gel + silver nanoparticles (AgNPs) (G+NPS), gel + exosomes (G+EXO) and gel + AgNPs + exosomes (Gel+NPS+EXO), at 14 days and, respectively, 21 days after the lesion. Tissue fragments were harvested and processed for histopathology and immunohistochemistry. Immunofluorescence was utilized to evaluate the maturity of underlaying granulation tissue based on double stainings for smooth muscle actin (SMA) and cluster of differentiation 31 (CD31). Our study showed variability in what it regards the vessel density and immunoexpression of SMA between the treatments, and image analysis revealed that most SMA reduction and blood vessel density reduction in the maturing granulation tissue occurred for the G+NPS and G+NPS+EXO treatments. A complete re-epithelization was also observed for the G+NPS+EXO treatment. Overall, our results show that improved topic treatments promote faster re-epithelization and reparation of the dermis after skin burn lesions, providing thus an avenue for new treatments that aim both local recuperation and systemic infection prevention. Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest 2021 2021-09-27 /pmc/articles/PMC8597381/ /pubmed/34609418 http://dx.doi.org/10.47162/RJME.62.1.15 Text en Copyright © 2020, Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited. |
spellingShingle | Original Paper Liliac, Ilona Mihaela Popescu, Elena Leocadia Văduva, Ion Alexandru Pirici, Daniel Mogoşanu, George Dan Streba, Costin Teodor Busuioc, Cristina Jana Bejenaru, Ludovic Everard Bejenaru, Cornelia Crăciunoiu, Nicolae Dumitru, Ilie Elayan, Hazim Mogoantă, Laurenţiu Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
title | Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
title_full | Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
title_fullStr | Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
title_full_unstemmed | Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
title_short | Nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
title_sort | nanoparticle-functionalized dressings for the treatment of third-degree skin burns – histopathological and immunohistochemical study |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597381/ https://www.ncbi.nlm.nih.gov/pubmed/34609418 http://dx.doi.org/10.47162/RJME.62.1.15 |
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