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TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum

B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percent...

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Autores principales: Wei, Haixia, Xie, Hongyan, Qu, Jiale, Xie, Anqi, Xie, Shihao, Huang, He, Li, Jiajie, Fang, Chao, Shi, Feihu, Qiu, Huaina, Qi, Yanwei, Tian, Xu, Yang, Quan, Huang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598019/
https://www.ncbi.nlm.nih.gov/pubmed/34788282
http://dx.doi.org/10.1371/journal.pntd.0009943
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author Wei, Haixia
Xie, Hongyan
Qu, Jiale
Xie, Anqi
Xie, Shihao
Huang, He
Li, Jiajie
Fang, Chao
Shi, Feihu
Qiu, Huaina
Qi, Yanwei
Tian, Xu
Yang, Quan
Huang, Jun
author_facet Wei, Haixia
Xie, Hongyan
Qu, Jiale
Xie, Anqi
Xie, Shihao
Huang, He
Li, Jiajie
Fang, Chao
Shi, Feihu
Qiu, Huaina
Qi, Yanwei
Tian, Xu
Yang, Quan
Huang, Jun
author_sort Wei, Haixia
collection PubMed
description B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice.
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spelling pubmed-85980192021-11-18 TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum Wei, Haixia Xie, Hongyan Qu, Jiale Xie, Anqi Xie, Shihao Huang, He Li, Jiajie Fang, Chao Shi, Feihu Qiu, Huaina Qi, Yanwei Tian, Xu Yang, Quan Huang, Jun PLoS Negl Trop Dis Research Article B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice. Public Library of Science 2021-11-17 /pmc/articles/PMC8598019/ /pubmed/34788282 http://dx.doi.org/10.1371/journal.pntd.0009943 Text en © 2021 Wei et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wei, Haixia
Xie, Hongyan
Qu, Jiale
Xie, Anqi
Xie, Shihao
Huang, He
Li, Jiajie
Fang, Chao
Shi, Feihu
Qiu, Huaina
Qi, Yanwei
Tian, Xu
Yang, Quan
Huang, Jun
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_full TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_fullStr TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_full_unstemmed TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_short TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
title_sort tlr7 modulating b-cell immune responses in the spleen of c57bl/6 mice infected with schistosoma japonicum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598019/
https://www.ncbi.nlm.nih.gov/pubmed/34788282
http://dx.doi.org/10.1371/journal.pntd.0009943
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