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Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold

PURPOSE: To evaluate methods that improve adipose-derived stem cells (ASCs) population in decellularized biological venous scaffold for tissue engineering in blood vessels, a model in rabbits. METHODS: The ASC was expanded until the third passage. Inferior vena cava (IVC) was submitted to the decell...

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Autores principales: Rodrigues, Lenize da Silva, Bovolato, Ana Lívia de Carvalho, Silva, Bárbara Esteves, Chizzolini, Leticia Victória, da Cruz, Bianca Latance, Moraes, Marcelo Padovani de Toledo, Lourenção, Pedro Luiz Toledo de Arruda, Bertanha, Matheus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598214/
https://www.ncbi.nlm.nih.gov/pubmed/34755757
http://dx.doi.org/10.1590/ACB361001
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author Rodrigues, Lenize da Silva
Bovolato, Ana Lívia de Carvalho
Silva, Bárbara Esteves
Chizzolini, Leticia Victória
da Cruz, Bianca Latance
Moraes, Marcelo Padovani de Toledo
Lourenção, Pedro Luiz Toledo de Arruda
Bertanha, Matheus
author_facet Rodrigues, Lenize da Silva
Bovolato, Ana Lívia de Carvalho
Silva, Bárbara Esteves
Chizzolini, Leticia Victória
da Cruz, Bianca Latance
Moraes, Marcelo Padovani de Toledo
Lourenção, Pedro Luiz Toledo de Arruda
Bertanha, Matheus
author_sort Rodrigues, Lenize da Silva
collection PubMed
description PURPOSE: To evaluate methods that improve adipose-derived stem cells (ASCs) population in decellularized biological venous scaffold for tissue engineering in blood vessels, a model in rabbits. METHODS: The ASC was expanded until the third passage. Inferior vena cava (IVC) was submitted to the decellularization process using 1% sodium dodecyl sulfate (SDS) or 2% sodium deoxycholate (SD) to compose 12 study groups (G): pure SD or SDS, exposed or not to 1% TritonX-100 (TX-100) and exposed or not to poly-l’lysine and laminin (PL). Scaffolds were covered with 1 × 10(5) or 1 × 10(6) ASCs diluted in 10 μL Puramatrix™. The histological analysis was done by cell counting in hematoxylin and eosin (HE) and nuclei count in immunofluorescence (IF) with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI). RESULTS: The study of groups in HE and IF showed similar results. For both analyses,IVC-SD-1 × 10(6) ASC and IVC-SD-PL-1 × 10(6) ASC provided the best results. The IF technique showed better sensitivity than HE, with a weak agreement between them. CONCLUSIONS: Decellularizing agent and the number of ASC influence scaffolds cellularization response and the best protocols as those ones using SD with or without the addition of PL.
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spelling pubmed-85982142021-11-18 Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold Rodrigues, Lenize da Silva Bovolato, Ana Lívia de Carvalho Silva, Bárbara Esteves Chizzolini, Leticia Victória da Cruz, Bianca Latance Moraes, Marcelo Padovani de Toledo Lourenção, Pedro Luiz Toledo de Arruda Bertanha, Matheus Acta Cir Bras Original Article PURPOSE: To evaluate methods that improve adipose-derived stem cells (ASCs) population in decellularized biological venous scaffold for tissue engineering in blood vessels, a model in rabbits. METHODS: The ASC was expanded until the third passage. Inferior vena cava (IVC) was submitted to the decellularization process using 1% sodium dodecyl sulfate (SDS) or 2% sodium deoxycholate (SD) to compose 12 study groups (G): pure SD or SDS, exposed or not to 1% TritonX-100 (TX-100) and exposed or not to poly-l’lysine and laminin (PL). Scaffolds were covered with 1 × 10(5) or 1 × 10(6) ASCs diluted in 10 μL Puramatrix™. The histological analysis was done by cell counting in hematoxylin and eosin (HE) and nuclei count in immunofluorescence (IF) with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI). RESULTS: The study of groups in HE and IF showed similar results. For both analyses,IVC-SD-1 × 10(6) ASC and IVC-SD-PL-1 × 10(6) ASC provided the best results. The IF technique showed better sensitivity than HE, with a weak agreement between them. CONCLUSIONS: Decellularizing agent and the number of ASC influence scaffolds cellularization response and the best protocols as those ones using SD with or without the addition of PL. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021-11-05 /pmc/articles/PMC8598214/ /pubmed/34755757 http://dx.doi.org/10.1590/ACB361001 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rodrigues, Lenize da Silva
Bovolato, Ana Lívia de Carvalho
Silva, Bárbara Esteves
Chizzolini, Leticia Victória
da Cruz, Bianca Latance
Moraes, Marcelo Padovani de Toledo
Lourenção, Pedro Luiz Toledo de Arruda
Bertanha, Matheus
Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
title Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
title_full Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
title_fullStr Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
title_full_unstemmed Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
title_short Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
title_sort quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598214/
https://www.ncbi.nlm.nih.gov/pubmed/34755757
http://dx.doi.org/10.1590/ACB361001
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