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Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2

The antiviral activity of recombinant human IFN-lambda type 1 (IFNλ-1) against culture strain of SARS-CoV-2 virus was determined by infecting a highly sensitive VeroE6 coronavirus cell culture after preincubation test (the cell monolayer was incubated with 4-fold dilutions of IFNλ-1 in a concentrati...

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Autores principales: Madonov, P. G., Svyatchenko, V. A., Legostaev, S. S., Kikhtenko, N. A., Kotlyarova, A. A., Oleinik, L. A., Baikalov, G. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598276/
https://www.ncbi.nlm.nih.gov/pubmed/34791556
http://dx.doi.org/10.1007/s10517-021-05330-0
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author Madonov, P. G.
Svyatchenko, V. A.
Legostaev, S. S.
Kikhtenko, N. A.
Kotlyarova, A. A.
Oleinik, L. A.
Baikalov, G. I.
author_facet Madonov, P. G.
Svyatchenko, V. A.
Legostaev, S. S.
Kikhtenko, N. A.
Kotlyarova, A. A.
Oleinik, L. A.
Baikalov, G. I.
author_sort Madonov, P. G.
collection PubMed
description The antiviral activity of recombinant human IFN-lambda type 1 (IFNλ-1) against culture strain of SARS-CoV-2 virus was determined by infecting a highly sensitive VeroE6 coronavirus cell culture after preincubation test (the cell monolayer was incubated with 4-fold dilutions of IFNλ-1 in a concentration range of 0.16-42,500 ng/ml in a culture medium for 12 h at 37°C) and without preincubation (simultaneous addition of different concentrations of IFNλ-1 and SARS-CoV-2 infection in a dose of 102 TCID50). The created recombinant human IFNλ-1 demonstrated obvious antiviral activity against SARS-CoV-2 virus in vitro. In the tests with and without preincubation, IFNλ-1 exhibited significant activity, although somewhat lower in variant with simultaneous addition of IFNλ-1 and virus to the cell culture. It should be noted that the antiviral effect of IFNλ-1 was observed in a wide range of concentrations.
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spelling pubmed-85982762021-11-18 Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2 Madonov, P. G. Svyatchenko, V. A. Legostaev, S. S. Kikhtenko, N. A. Kotlyarova, A. A. Oleinik, L. A. Baikalov, G. I. Bull Exp Biol Med Article The antiviral activity of recombinant human IFN-lambda type 1 (IFNλ-1) against culture strain of SARS-CoV-2 virus was determined by infecting a highly sensitive VeroE6 coronavirus cell culture after preincubation test (the cell monolayer was incubated with 4-fold dilutions of IFNλ-1 in a concentration range of 0.16-42,500 ng/ml in a culture medium for 12 h at 37°C) and without preincubation (simultaneous addition of different concentrations of IFNλ-1 and SARS-CoV-2 infection in a dose of 102 TCID50). The created recombinant human IFNλ-1 demonstrated obvious antiviral activity against SARS-CoV-2 virus in vitro. In the tests with and without preincubation, IFNλ-1 exhibited significant activity, although somewhat lower in variant with simultaneous addition of IFNλ-1 and virus to the cell culture. It should be noted that the antiviral effect of IFNλ-1 was observed in a wide range of concentrations. Springer US 2021-11-18 2021 /pmc/articles/PMC8598276/ /pubmed/34791556 http://dx.doi.org/10.1007/s10517-021-05330-0 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Madonov, P. G.
Svyatchenko, V. A.
Legostaev, S. S.
Kikhtenko, N. A.
Kotlyarova, A. A.
Oleinik, L. A.
Baikalov, G. I.
Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
title Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
title_full Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
title_fullStr Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
title_full_unstemmed Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
title_short Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
title_sort evaluation of the anti-viral activity of human recombinant interferon lambda-1 against sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598276/
https://www.ncbi.nlm.nih.gov/pubmed/34791556
http://dx.doi.org/10.1007/s10517-021-05330-0
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