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An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment

PURPOSE: The aim of the study is to examine the possible therapeutic effects of a known cardiac glycoside, digoxin, on a rat model of MTX-induced hepatotoxicity. METHODS: The study was conducted on twenty-four male rats. While eighteen rats received a single dose of 20 mg/kg MTX to obtain an injured...

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Autores principales: Taskin, Banu, Erdoğan, Mümin Alper, Yiğittürk, Gürkan, Alper, Sibel, Erbaş, Oytun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598353/
https://www.ncbi.nlm.nih.gov/pubmed/34804155
http://dx.doi.org/10.1155/2021/6619844
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author Taskin, Banu
Erdoğan, Mümin Alper
Yiğittürk, Gürkan
Alper, Sibel
Erbaş, Oytun
author_facet Taskin, Banu
Erdoğan, Mümin Alper
Yiğittürk, Gürkan
Alper, Sibel
Erbaş, Oytun
author_sort Taskin, Banu
collection PubMed
description PURPOSE: The aim of the study is to examine the possible therapeutic effects of a known cardiac glycoside, digoxin, on a rat model of MTX-induced hepatotoxicity. METHODS: The study was conducted on twenty-four male rats. While eighteen rats received a single dose of 20 mg/kg MTX to obtain an injured liver model, six rats constituted the control group. Also, the eighteen liver toxicity model created rats were equally divided into two groups, one of which received digoxin 0.1 mg/kg/day digoxin (Group 1) and the other group (Group 2) was given saline (% 0.9NaCl) with a dose of 1 ml/kg/day for ten days. Following the trial, the rats were sacrificed to harvest blood and liver tissue samples to determine blood and tissue MDA, serum ALT, plasma TNF-α, TGF-β, IL-6, IL-1-Beta, and PTX3 levels. RESULTS: MTX's structural and functional hepatotoxicity was observable and evidenced by relatively worse histopathological scores and increased biochemical marker levels. Digoxin treatment significantly reduced the liver enzyme ALT, plasma TNF-α, TGF-β, PTX3, and MDA levels and decreased histological changes in the liver tissue with MTX-induced hepatotoxicity in the rat model. CONCLUSION: We suggest that digoxin has an anti-inflammatory and antihepatotoxic effect on the MTX-induced liver injury model.
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spelling pubmed-85983532021-11-18 An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment Taskin, Banu Erdoğan, Mümin Alper Yiğittürk, Gürkan Alper, Sibel Erbaş, Oytun Gastroenterol Res Pract Research Article PURPOSE: The aim of the study is to examine the possible therapeutic effects of a known cardiac glycoside, digoxin, on a rat model of MTX-induced hepatotoxicity. METHODS: The study was conducted on twenty-four male rats. While eighteen rats received a single dose of 20 mg/kg MTX to obtain an injured liver model, six rats constituted the control group. Also, the eighteen liver toxicity model created rats were equally divided into two groups, one of which received digoxin 0.1 mg/kg/day digoxin (Group 1) and the other group (Group 2) was given saline (% 0.9NaCl) with a dose of 1 ml/kg/day for ten days. Following the trial, the rats were sacrificed to harvest blood and liver tissue samples to determine blood and tissue MDA, serum ALT, plasma TNF-α, TGF-β, IL-6, IL-1-Beta, and PTX3 levels. RESULTS: MTX's structural and functional hepatotoxicity was observable and evidenced by relatively worse histopathological scores and increased biochemical marker levels. Digoxin treatment significantly reduced the liver enzyme ALT, plasma TNF-α, TGF-β, PTX3, and MDA levels and decreased histological changes in the liver tissue with MTX-induced hepatotoxicity in the rat model. CONCLUSION: We suggest that digoxin has an anti-inflammatory and antihepatotoxic effect on the MTX-induced liver injury model. Hindawi 2021-11-10 /pmc/articles/PMC8598353/ /pubmed/34804155 http://dx.doi.org/10.1155/2021/6619844 Text en Copyright © 2021 Banu Taskin et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Taskin, Banu
Erdoğan, Mümin Alper
Yiğittürk, Gürkan
Alper, Sibel
Erbaş, Oytun
An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment
title An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment
title_full An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment
title_fullStr An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment
title_full_unstemmed An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment
title_short An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment
title_sort experimental study: benefits of digoxin on hepatotoxicity induced by methotrexate treatment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598353/
https://www.ncbi.nlm.nih.gov/pubmed/34804155
http://dx.doi.org/10.1155/2021/6619844
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