Cargando…

Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease

Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a neglected tropical disease and a health problem in Latin America. Etiological treatment has limited effectiveness in chronic CD; thus, new therapeutic strategies are required. The practice of physical exercises has been widely advo...

Descripción completa

Detalles Bibliográficos
Autores principales: Pedra-Rezende, Yasmin, Barbosa, Juliana M. C., Bombaça, Ana Cristina S., Dantas-Pereira, Luiza, Gibaldi, Daniel, Vilar-Pereira, Glaucia, dos Santos, Hílton Antônio Mata, Ramos, Isalira Peroba, Silva-Gomes, Natália Lins, Moreira, Otacilio C., Lannes-Vieira, Joseli, Menna-Barreto, Rubem F. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599157/
https://www.ncbi.nlm.nih.gov/pubmed/34804007
http://dx.doi.org/10.3389/fimmu.2021.712034
_version_ 1784600885740961792
author Pedra-Rezende, Yasmin
Barbosa, Juliana M. C.
Bombaça, Ana Cristina S.
Dantas-Pereira, Luiza
Gibaldi, Daniel
Vilar-Pereira, Glaucia
dos Santos, Hílton Antônio Mata
Ramos, Isalira Peroba
Silva-Gomes, Natália Lins
Moreira, Otacilio C.
Lannes-Vieira, Joseli
Menna-Barreto, Rubem F. S.
author_facet Pedra-Rezende, Yasmin
Barbosa, Juliana M. C.
Bombaça, Ana Cristina S.
Dantas-Pereira, Luiza
Gibaldi, Daniel
Vilar-Pereira, Glaucia
dos Santos, Hílton Antônio Mata
Ramos, Isalira Peroba
Silva-Gomes, Natália Lins
Moreira, Otacilio C.
Lannes-Vieira, Joseli
Menna-Barreto, Rubem F. S.
author_sort Pedra-Rezende, Yasmin
collection PubMed
description Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a neglected tropical disease and a health problem in Latin America. Etiological treatment has limited effectiveness in chronic CD; thus, new therapeutic strategies are required. The practice of physical exercises has been widely advocated to improve the quality of life of CD patients. The most frequent clinical CD manifestation is the chronic indeterminate form (CIF), and the effect of physical exercises on disease progression remains unknown. Here, in a CIF model, we aimed to evaluate the effect of physical exercises on cardiac histological, parasitological, mitochondrial, and oxidative metabolism, electro and echocardiographic profiles, and immunological features. To establish a CIF model, BALB/c and C57BL/6 mice were infected with 100 and 500 trypomastigotes of the Y T. cruzi strain. At 120 days postinfection (dpi), all mouse groups showed normal PR and corrected QT intervals and QRS complexes. Compared to BALB/c mice, C57BL/6 mice showed a lower parasitemia peak, mortality rate, and less intense myocarditis. Thus, C57BL/6 mice infected with 500 parasites were used for subsequent analyses. At 120 dpi, a decrease in cardiac mitochondrial oxygen consumption and an increase in reactive oxygen species (ROS) were detected. When we increased the number of analyzed mice, a reduced heart rate and slightly prolonged corrected QT intervals were detected, at 120 and 150 dpi, which were then normalized at 180 dpi, thus characterizing the CIF. Y-infected mice were subjected to an exercise program on a treadmill for 4 weeks (from 150 to 180 dpi), five times per week in a 30–60-min daily training session. At 180 dpi, no alterations were detected in cardiac mitochondrial and oxidative metabolism, which were not affected by physical exercises, although ROS production increased. At 120 and 180 dpi, comparing infected and non-infected mice, no differences were observed in the levels of plasma cytokines, indicating that a crucial biomarker of the systemic inflammatory profile was absent and not affected by exercise. Compared with sedentary mice, trained Y-infected mice showed similar parasite loads and inflammatory cells but reduced cardiac fibrosis. Therefore, our data show that physical exercises promote beneficial changes that may prevent CD progression.
format Online
Article
Text
id pubmed-8599157
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85991572021-11-19 Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease Pedra-Rezende, Yasmin Barbosa, Juliana M. C. Bombaça, Ana Cristina S. Dantas-Pereira, Luiza Gibaldi, Daniel Vilar-Pereira, Glaucia dos Santos, Hílton Antônio Mata Ramos, Isalira Peroba Silva-Gomes, Natália Lins Moreira, Otacilio C. Lannes-Vieira, Joseli Menna-Barreto, Rubem F. S. Front Immunol Immunology Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a neglected tropical disease and a health problem in Latin America. Etiological treatment has limited effectiveness in chronic CD; thus, new therapeutic strategies are required. The practice of physical exercises has been widely advocated to improve the quality of life of CD patients. The most frequent clinical CD manifestation is the chronic indeterminate form (CIF), and the effect of physical exercises on disease progression remains unknown. Here, in a CIF model, we aimed to evaluate the effect of physical exercises on cardiac histological, parasitological, mitochondrial, and oxidative metabolism, electro and echocardiographic profiles, and immunological features. To establish a CIF model, BALB/c and C57BL/6 mice were infected with 100 and 500 trypomastigotes of the Y T. cruzi strain. At 120 days postinfection (dpi), all mouse groups showed normal PR and corrected QT intervals and QRS complexes. Compared to BALB/c mice, C57BL/6 mice showed a lower parasitemia peak, mortality rate, and less intense myocarditis. Thus, C57BL/6 mice infected with 500 parasites were used for subsequent analyses. At 120 dpi, a decrease in cardiac mitochondrial oxygen consumption and an increase in reactive oxygen species (ROS) were detected. When we increased the number of analyzed mice, a reduced heart rate and slightly prolonged corrected QT intervals were detected, at 120 and 150 dpi, which were then normalized at 180 dpi, thus characterizing the CIF. Y-infected mice were subjected to an exercise program on a treadmill for 4 weeks (from 150 to 180 dpi), five times per week in a 30–60-min daily training session. At 180 dpi, no alterations were detected in cardiac mitochondrial and oxidative metabolism, which were not affected by physical exercises, although ROS production increased. At 120 and 180 dpi, comparing infected and non-infected mice, no differences were observed in the levels of plasma cytokines, indicating that a crucial biomarker of the systemic inflammatory profile was absent and not affected by exercise. Compared with sedentary mice, trained Y-infected mice showed similar parasite loads and inflammatory cells but reduced cardiac fibrosis. Therefore, our data show that physical exercises promote beneficial changes that may prevent CD progression. Frontiers Media S.A. 2021-11-04 /pmc/articles/PMC8599157/ /pubmed/34804007 http://dx.doi.org/10.3389/fimmu.2021.712034 Text en Copyright © 2021 Pedra-Rezende, Barbosa, Bombaça, Dantas-Pereira, Gibaldi, Vilar-Pereira, dos Santos, Ramos, Silva-Gomes, Moreira, Lannes-Vieira and Menna-Barreto https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Pedra-Rezende, Yasmin
Barbosa, Juliana M. C.
Bombaça, Ana Cristina S.
Dantas-Pereira, Luiza
Gibaldi, Daniel
Vilar-Pereira, Glaucia
dos Santos, Hílton Antônio Mata
Ramos, Isalira Peroba
Silva-Gomes, Natália Lins
Moreira, Otacilio C.
Lannes-Vieira, Joseli
Menna-Barreto, Rubem F. S.
Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
title Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
title_full Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
title_fullStr Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
title_full_unstemmed Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
title_short Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
title_sort physical exercise promotes a reduction in cardiac fibrosis in the chronic indeterminate form of experimental chagas disease
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599157/
https://www.ncbi.nlm.nih.gov/pubmed/34804007
http://dx.doi.org/10.3389/fimmu.2021.712034
work_keys_str_mv AT pedrarezendeyasmin physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT barbosajulianamc physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT bombacaanacristinas physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT dantaspereiraluiza physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT gibaldidaniel physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT vilarpereiraglaucia physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT dossantoshiltonantoniomata physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT ramosisaliraperoba physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT silvagomesnatalialins physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT moreiraotacilioc physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT lannesvieirajoseli physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease
AT mennabarretorubemfs physicalexercisepromotesareductionincardiacfibrosisinthechronicindeterminateformofexperimentalchagasdisease