Cargando…

Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis

Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), but there is still a lack of effective therapy. Multiple studies have reported that endoplasmic reticulum (ER) stress and extracellular matrix (ECM) degradation exert an enormous function on the occurrence and developmen...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Zhen, Ni, Libin, Teng, Cheng, Zhang, Zhao, Wu, Long, Jin, Yu, Lu, Xinlei, Lin, Zhongke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599281/
https://www.ncbi.nlm.nih.gov/pubmed/34805156
http://dx.doi.org/10.3389/fcell.2021.745621
_version_ 1784600914430001152
author Lin, Zhen
Ni, Libin
Teng, Cheng
Zhang, Zhao
Wu, Long
Jin, Yu
Lu, Xinlei
Lin, Zhongke
author_facet Lin, Zhen
Ni, Libin
Teng, Cheng
Zhang, Zhao
Wu, Long
Jin, Yu
Lu, Xinlei
Lin, Zhongke
author_sort Lin, Zhen
collection PubMed
description Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), but there is still a lack of effective therapy. Multiple studies have reported that endoplasmic reticulum (ER) stress and extracellular matrix (ECM) degradation exert an enormous function on the occurrence and development of IDD. Autophagy can effectively repair ER stress and maintain ECM homeostasis. Eicosapentaenoic acid (EPA) can specifically induce autophagy. The purpose of this study is to demonstrate that EPA can promote autophagy, reduce ECM degradation and ER stress in vitro, thereby reducing cell apoptosis, and the protective effects of EPA in an IDD-rat model in vivo. Western blot and immunofluorescence were used to detect the autophagic flux, ER stress, ECM degradation, and apoptosis in nucleus pulposus cells (NPCs) treated by EPA. We also used puncture-induced IDD rats as experimental subjects to observe the therapeutic effect of EPA on IDD. Our findings indicated that EPA can effectively improve the autophagy activity in NPCs, inhibit the endoplasmic reticulum stress process, reduce the degree of cell apoptosis, and exert protective effects on the anabolism and catabolism of ECM. In addition, in vivo investigations demonstrated that EPA ameliorated the progression of puncture-induced IDD in rats. In conclusion, this study revealed the intrinsic mechanisms of EPA’s protective role in NPCs and its potential therapeutic significance for the treatment of IDD.
format Online
Article
Text
id pubmed-8599281
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85992812021-11-19 Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis Lin, Zhen Ni, Libin Teng, Cheng Zhang, Zhao Wu, Long Jin, Yu Lu, Xinlei Lin, Zhongke Front Cell Dev Biol Cell and Developmental Biology Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), but there is still a lack of effective therapy. Multiple studies have reported that endoplasmic reticulum (ER) stress and extracellular matrix (ECM) degradation exert an enormous function on the occurrence and development of IDD. Autophagy can effectively repair ER stress and maintain ECM homeostasis. Eicosapentaenoic acid (EPA) can specifically induce autophagy. The purpose of this study is to demonstrate that EPA can promote autophagy, reduce ECM degradation and ER stress in vitro, thereby reducing cell apoptosis, and the protective effects of EPA in an IDD-rat model in vivo. Western blot and immunofluorescence were used to detect the autophagic flux, ER stress, ECM degradation, and apoptosis in nucleus pulposus cells (NPCs) treated by EPA. We also used puncture-induced IDD rats as experimental subjects to observe the therapeutic effect of EPA on IDD. Our findings indicated that EPA can effectively improve the autophagy activity in NPCs, inhibit the endoplasmic reticulum stress process, reduce the degree of cell apoptosis, and exert protective effects on the anabolism and catabolism of ECM. In addition, in vivo investigations demonstrated that EPA ameliorated the progression of puncture-induced IDD in rats. In conclusion, this study revealed the intrinsic mechanisms of EPA’s protective role in NPCs and its potential therapeutic significance for the treatment of IDD. Frontiers Media S.A. 2021-11-04 /pmc/articles/PMC8599281/ /pubmed/34805156 http://dx.doi.org/10.3389/fcell.2021.745621 Text en Copyright © 2021 Lin, Ni, Teng, Zhang, Wu, Jin, Lu and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Lin, Zhen
Ni, Libin
Teng, Cheng
Zhang, Zhao
Wu, Long
Jin, Yu
Lu, Xinlei
Lin, Zhongke
Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis
title Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis
title_full Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis
title_fullStr Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis
title_full_unstemmed Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis
title_short Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis
title_sort eicosapentaenoic acid-induced autophagy attenuates intervertebral disc degeneration by suppressing endoplasmic reticulum stress, extracellular matrix degradation, and apoptosis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599281/
https://www.ncbi.nlm.nih.gov/pubmed/34805156
http://dx.doi.org/10.3389/fcell.2021.745621
work_keys_str_mv AT linzhen eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT nilibin eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT tengcheng eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT zhangzhao eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT wulong eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT jinyu eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT luxinlei eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis
AT linzhongke eicosapentaenoicacidinducedautophagyattenuatesintervertebraldiscdegenerationbysuppressingendoplasmicreticulumstressextracellularmatrixdegradationandapoptosis