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A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia

Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget met...

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Autores principales: Liu, Zheng, Huo, Jin-hai, Dong, Wen-ting, Sun, Guo-dong, Li, Feng-jin, Zhang, Ya-nan, Qin, Zhi-wei, pengna, Jiang, Wang, Wei-ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599429/
https://www.ncbi.nlm.nih.gov/pubmed/34803705
http://dx.doi.org/10.3389/fphar.2021.761883
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author Liu, Zheng
Huo, Jin-hai
Dong, Wen-ting
Sun, Guo-dong
Li, Feng-jin
Zhang, Ya-nan
Qin, Zhi-wei
pengna, Jiang
Wang, Wei-ming
author_facet Liu, Zheng
Huo, Jin-hai
Dong, Wen-ting
Sun, Guo-dong
Li, Feng-jin
Zhang, Ya-nan
Qin, Zhi-wei
pengna, Jiang
Wang, Wei-ming
author_sort Liu, Zheng
collection PubMed
description Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget metabolomics studies were performed on rat serum, urine, and lung tissues, and 56 therapeutic biomarkers were found. Subsequently, the components of QB absorbed into the blood and lung tissues were clarified, and based on this finding, the core target of network pharmacology was predicted. The enrichment analysis of biomarkers–genes finally confirmed their close relationship with the NF-κB signaling pathway. By western blotting expression of the proteins in the lung tissue–related signaling pathways, it is finally confirmed that QB inhibits the NF-κB signaling pathway through SIRT1, IL-10 and MMP9, CTNNB1, EGFR, and other targets. It plays a role in regulating immunity, regulating metabolism, and treating diseases.
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spelling pubmed-85994292021-11-19 A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia Liu, Zheng Huo, Jin-hai Dong, Wen-ting Sun, Guo-dong Li, Feng-jin Zhang, Ya-nan Qin, Zhi-wei pengna, Jiang Wang, Wei-ming Front Pharmacol Pharmacology Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget metabolomics studies were performed on rat serum, urine, and lung tissues, and 56 therapeutic biomarkers were found. Subsequently, the components of QB absorbed into the blood and lung tissues were clarified, and based on this finding, the core target of network pharmacology was predicted. The enrichment analysis of biomarkers–genes finally confirmed their close relationship with the NF-κB signaling pathway. By western blotting expression of the proteins in the lung tissue–related signaling pathways, it is finally confirmed that QB inhibits the NF-κB signaling pathway through SIRT1, IL-10 and MMP9, CTNNB1, EGFR, and other targets. It plays a role in regulating immunity, regulating metabolism, and treating diseases. Frontiers Media S.A. 2021-11-04 /pmc/articles/PMC8599429/ /pubmed/34803705 http://dx.doi.org/10.3389/fphar.2021.761883 Text en Copyright © 2021 Liu, Huo, Dong, Sun, Li, Zhang, Qin, pengna and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Liu, Zheng
Huo, Jin-hai
Dong, Wen-ting
Sun, Guo-dong
Li, Feng-jin
Zhang, Ya-nan
Qin, Zhi-wei
pengna, Jiang
Wang, Wei-ming
A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
title A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
title_full A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
title_fullStr A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
title_full_unstemmed A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
title_short A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
title_sort study based on metabolomics, network pharmacology, and experimental verification to explore the mechanism of qinbaiqingfei concentrated pills in the treatment of mycoplasma pneumonia
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599429/
https://www.ncbi.nlm.nih.gov/pubmed/34803705
http://dx.doi.org/10.3389/fphar.2021.761883
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