Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical...

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Autores principales: Yu, Che-Hang, Stirman, Jeffrey N., Yu, Yiyi, Hira, Riichiro, Smith, Spencer L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599518/
https://www.ncbi.nlm.nih.gov/pubmed/34789723
http://dx.doi.org/10.1038/s41467-021-26736-4
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author Yu, Che-Hang
Stirman, Jeffrey N.
Yu, Yiyi
Hira, Riichiro
Smith, Spencer L.
author_facet Yu, Che-Hang
Stirman, Jeffrey N.
Yu, Yiyi
Hira, Riichiro
Smith, Spencer L.
author_sort Yu, Che-Hang
collection PubMed
description Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm(2), encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.
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spelling pubmed-85995182021-11-19 Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry Yu, Che-Hang Stirman, Jeffrey N. Yu, Yiyi Hira, Riichiro Smith, Spencer L. Nat Commun Article Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm(2), encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain. Nature Publishing Group UK 2021-11-17 /pmc/articles/PMC8599518/ /pubmed/34789723 http://dx.doi.org/10.1038/s41467-021-26736-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yu, Che-Hang
Stirman, Jeffrey N.
Yu, Yiyi
Hira, Riichiro
Smith, Spencer L.
Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
title Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
title_full Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
title_fullStr Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
title_full_unstemmed Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
title_short Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
title_sort diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599518/
https://www.ncbi.nlm.nih.gov/pubmed/34789723
http://dx.doi.org/10.1038/s41467-021-26736-4
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