Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous vid...

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Autores principales: Clough, Mitchell, Chen, Ichun Anderson, Park, Seong-Wook, Ahrens, Allison M., Stirman, Jeffrey N., Smith, Spencer L., Chen, Jerry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599611/
https://www.ncbi.nlm.nih.gov/pubmed/34789730
http://dx.doi.org/10.1038/s41467-021-26737-3
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author Clough, Mitchell
Chen, Ichun Anderson
Park, Seong-Wook
Ahrens, Allison M.
Stirman, Jeffrey N.
Smith, Spencer L.
Chen, Jerry L.
author_facet Clough, Mitchell
Chen, Ichun Anderson
Park, Seong-Wook
Ahrens, Allison M.
Stirman, Jeffrey N.
Smith, Spencer L.
Chen, Jerry L.
author_sort Clough, Mitchell
collection PubMed
description Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.
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spelling pubmed-85996112021-11-19 Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds Clough, Mitchell Chen, Ichun Anderson Park, Seong-Wook Ahrens, Allison M. Stirman, Jeffrey N. Smith, Spencer L. Chen, Jerry L. Nat Commun Article Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales. Nature Publishing Group UK 2021-11-17 /pmc/articles/PMC8599611/ /pubmed/34789730 http://dx.doi.org/10.1038/s41467-021-26737-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Clough, Mitchell
Chen, Ichun Anderson
Park, Seong-Wook
Ahrens, Allison M.
Stirman, Jeffrey N.
Smith, Spencer L.
Chen, Jerry L.
Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
title Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
title_full Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
title_fullStr Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
title_full_unstemmed Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
title_short Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
title_sort flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599611/
https://www.ncbi.nlm.nih.gov/pubmed/34789730
http://dx.doi.org/10.1038/s41467-021-26737-3
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