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Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
The kidney is a complex organ composed of more than 30 terminally differentiated cell types that all are required to perform its numerous homeostatic functions. Defects in kidney development are a significant cause of chronic kidney disease in children, which can lead to kidney failure that can only...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599654/ https://www.ncbi.nlm.nih.gov/pubmed/34789782 http://dx.doi.org/10.1038/s41598-021-01790-6 |
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author | Bais, Abha S. Cerqueira, Débora M. Clugston, Andrew Bodnar, Andrew J. Ho, Jacqueline Kostka, Dennis |
author_facet | Bais, Abha S. Cerqueira, Débora M. Clugston, Andrew Bodnar, Andrew J. Ho, Jacqueline Kostka, Dennis |
author_sort | Bais, Abha S. |
collection | PubMed |
description | The kidney is a complex organ composed of more than 30 terminally differentiated cell types that all are required to perform its numerous homeostatic functions. Defects in kidney development are a significant cause of chronic kidney disease in children, which can lead to kidney failure that can only be treated by transplant or dialysis. A better understanding of molecular mechanisms that drive kidney development is important for designing strategies to enhance renal repair and regeneration. In this study, we profiled gene expression in the developing mouse kidney at embryonic day 14.5 at single-cell resolution. Consistent with previous studies, clusters with distinct transcriptional signatures clearly identify major compartments and cell types of the developing kidney. Cell cycle activity distinguishes between the “primed” and “self-renewing” sub-populations of nephron progenitors, with increased expression of the cell cycle-related genes Birc5, Cdca3, Smc2 and Smc4 in “primed” nephron progenitors. In addition, augmented expression of cell cycle related genes Birc5, Cks2, Ccnb1, Ccnd1 and Tuba1a/b was detected in immature distal tubules, suggesting cell cycle regulation may be required for early events of nephron patterning and tubular fusion between the distal nephron and collecting duct epithelia. |
format | Online Article Text |
id | pubmed-8599654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85996542021-11-19 Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis Bais, Abha S. Cerqueira, Débora M. Clugston, Andrew Bodnar, Andrew J. Ho, Jacqueline Kostka, Dennis Sci Rep Article The kidney is a complex organ composed of more than 30 terminally differentiated cell types that all are required to perform its numerous homeostatic functions. Defects in kidney development are a significant cause of chronic kidney disease in children, which can lead to kidney failure that can only be treated by transplant or dialysis. A better understanding of molecular mechanisms that drive kidney development is important for designing strategies to enhance renal repair and regeneration. In this study, we profiled gene expression in the developing mouse kidney at embryonic day 14.5 at single-cell resolution. Consistent with previous studies, clusters with distinct transcriptional signatures clearly identify major compartments and cell types of the developing kidney. Cell cycle activity distinguishes between the “primed” and “self-renewing” sub-populations of nephron progenitors, with increased expression of the cell cycle-related genes Birc5, Cdca3, Smc2 and Smc4 in “primed” nephron progenitors. In addition, augmented expression of cell cycle related genes Birc5, Cks2, Ccnb1, Ccnd1 and Tuba1a/b was detected in immature distal tubules, suggesting cell cycle regulation may be required for early events of nephron patterning and tubular fusion between the distal nephron and collecting duct epithelia. Nature Publishing Group UK 2021-11-17 /pmc/articles/PMC8599654/ /pubmed/34789782 http://dx.doi.org/10.1038/s41598-021-01790-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bais, Abha S. Cerqueira, Débora M. Clugston, Andrew Bodnar, Andrew J. Ho, Jacqueline Kostka, Dennis Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
title | Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
title_full | Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
title_fullStr | Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
title_full_unstemmed | Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
title_short | Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
title_sort | single-cell rna sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599654/ https://www.ncbi.nlm.nih.gov/pubmed/34789782 http://dx.doi.org/10.1038/s41598-021-01790-6 |
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