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DEspR(high) neutrophils are associated with critical illness in COVID-19
SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities for thera...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599677/ https://www.ncbi.nlm.nih.gov/pubmed/34789851 http://dx.doi.org/10.1038/s41598-021-01943-7 |
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author | deKay, Joanne T. Emery, Ivette F. Rud, Jonathan Eldridge, Ashley Lord, Christine Gagnon, David J. May, Teresa L. Herrera, Victoria L. M. Ruiz-Opazo, Nelson Riker, Richard R. Sawyer, Douglas B. Ryzhov, Sergey Seder, David B. |
author_facet | deKay, Joanne T. Emery, Ivette F. Rud, Jonathan Eldridge, Ashley Lord, Christine Gagnon, David J. May, Teresa L. Herrera, Victoria L. M. Ruiz-Opazo, Nelson Riker, Richard R. Sawyer, Douglas B. Ryzhov, Sergey Seder, David B. |
author_sort | deKay, Joanne T. |
collection | PubMed |
description | SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities for therapy. We studied immune cell subpopulations and their associations with clinical parameters in a cohort of 26 patients with COVID-19. Following informed consent, we collected blood samples from hospitalized patients with COVID-19 within 72 h of admission. Flow cytometry was used to analyze white blood cell subpopulations. Plasma levels of cytokines and chemokines were measured using ELISA. Neutrophils undergoing neutrophil extracellular traps (NET) formation were evaluated in blood smears. We examined the immunophenotype of patients with COVID-19 in comparison to that of SARS-CoV-2 negative controls. A novel subset of pro-inflammatory neutrophils expressing a high level of dual endothelin-1 and VEGF signal peptide-activated receptor (DEspR) at the cell surface was found to be associated with elevated circulating CCL23, increased NETosis, and critical-severity COVID-19 illness. The potential to target this subpopulation of neutrophils to reduce secondary tissue damage caused by SARS-CoV-2 infection warrants further investigation. |
format | Online Article Text |
id | pubmed-8599677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85996772021-11-19 DEspR(high) neutrophils are associated with critical illness in COVID-19 deKay, Joanne T. Emery, Ivette F. Rud, Jonathan Eldridge, Ashley Lord, Christine Gagnon, David J. May, Teresa L. Herrera, Victoria L. M. Ruiz-Opazo, Nelson Riker, Richard R. Sawyer, Douglas B. Ryzhov, Sergey Seder, David B. Sci Rep Article SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities for therapy. We studied immune cell subpopulations and their associations with clinical parameters in a cohort of 26 patients with COVID-19. Following informed consent, we collected blood samples from hospitalized patients with COVID-19 within 72 h of admission. Flow cytometry was used to analyze white blood cell subpopulations. Plasma levels of cytokines and chemokines were measured using ELISA. Neutrophils undergoing neutrophil extracellular traps (NET) formation were evaluated in blood smears. We examined the immunophenotype of patients with COVID-19 in comparison to that of SARS-CoV-2 negative controls. A novel subset of pro-inflammatory neutrophils expressing a high level of dual endothelin-1 and VEGF signal peptide-activated receptor (DEspR) at the cell surface was found to be associated with elevated circulating CCL23, increased NETosis, and critical-severity COVID-19 illness. The potential to target this subpopulation of neutrophils to reduce secondary tissue damage caused by SARS-CoV-2 infection warrants further investigation. Nature Publishing Group UK 2021-11-17 /pmc/articles/PMC8599677/ /pubmed/34789851 http://dx.doi.org/10.1038/s41598-021-01943-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article deKay, Joanne T. Emery, Ivette F. Rud, Jonathan Eldridge, Ashley Lord, Christine Gagnon, David J. May, Teresa L. Herrera, Victoria L. M. Ruiz-Opazo, Nelson Riker, Richard R. Sawyer, Douglas B. Ryzhov, Sergey Seder, David B. DEspR(high) neutrophils are associated with critical illness in COVID-19 |
title | DEspR(high) neutrophils are associated with critical illness in COVID-19 |
title_full | DEspR(high) neutrophils are associated with critical illness in COVID-19 |
title_fullStr | DEspR(high) neutrophils are associated with critical illness in COVID-19 |
title_full_unstemmed | DEspR(high) neutrophils are associated with critical illness in COVID-19 |
title_short | DEspR(high) neutrophils are associated with critical illness in COVID-19 |
title_sort | despr(high) neutrophils are associated with critical illness in covid-19 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599677/ https://www.ncbi.nlm.nih.gov/pubmed/34789851 http://dx.doi.org/10.1038/s41598-021-01943-7 |
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