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Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae

When presented with the choice, Drosophila melanogaster females will often prefer to lay eggs on food containing a significant amount of alcohol. While, in some cases, this behavioral decision can provide a survival advantage to the developing larvae, it can also lead to developmental and cognitive...

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Autores principales: Anqueira-González, Amanda, Acevedo-Gonzalez, Jenny P., Montes-Mercado, Airined, Irizarry-Hernández, Claudia, Fuenzalida-Uribe, Nicolás L., Ghezzi, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599819/
https://www.ncbi.nlm.nih.gov/pubmed/34803626
http://dx.doi.org/10.3389/fnbeh.2021.768694
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author Anqueira-González, Amanda
Acevedo-Gonzalez, Jenny P.
Montes-Mercado, Airined
Irizarry-Hernández, Claudia
Fuenzalida-Uribe, Nicolás L.
Ghezzi, Alfredo
author_facet Anqueira-González, Amanda
Acevedo-Gonzalez, Jenny P.
Montes-Mercado, Airined
Irizarry-Hernández, Claudia
Fuenzalida-Uribe, Nicolás L.
Ghezzi, Alfredo
author_sort Anqueira-González, Amanda
collection PubMed
description When presented with the choice, Drosophila melanogaster females will often prefer to lay eggs on food containing a significant amount of alcohol. While, in some cases, this behavioral decision can provide a survival advantage to the developing larvae, it can also lead to developmental and cognitive problems. Alcohol consumption can affect executive functions, episodic memory, and other brain function capacities. However, in the fruit fly, the initial cognitive effects of alcohol consumption have been shown to reverse upon persistent exposure to alcohol. Using an olfactory conditioning assay where an odorant is implemented as a conditioned stimulus and paired with a heat shock as an unconditioned stimulus, a previous study has shown that when exposed to a short acute dose of alcohol, Drosophila larvae can no longer learn this association. Interestingly, upon prolonged chronic alcohol exposure, larvae seem to successfully avoid the conditioned stimulus just as well as control alcohol-naive larvae, suggestive of alcohol-induced neuroadaptations. However, the mechanisms by which Drosophila adapt to the presence of alcohol remains unknown. In this study, we explore the transcriptional correlates of neuroadaptation in Drosophila larvae exposed to chronic alcohol to understand the genetic and cellular components responsible for this adaptation. For this, we employed RNA sequencing technology to evaluate differences in gene expression in the brain of larvae chronically exposed to alcohol. Our results suggest that alcohol-induced neuroadaptations are modulated by a diverse array of synaptic genes within the larval brain through a series of epigenetic modulators.
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spelling pubmed-85998192021-11-19 Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae Anqueira-González, Amanda Acevedo-Gonzalez, Jenny P. Montes-Mercado, Airined Irizarry-Hernández, Claudia Fuenzalida-Uribe, Nicolás L. Ghezzi, Alfredo Front Behav Neurosci Neuroscience When presented with the choice, Drosophila melanogaster females will often prefer to lay eggs on food containing a significant amount of alcohol. While, in some cases, this behavioral decision can provide a survival advantage to the developing larvae, it can also lead to developmental and cognitive problems. Alcohol consumption can affect executive functions, episodic memory, and other brain function capacities. However, in the fruit fly, the initial cognitive effects of alcohol consumption have been shown to reverse upon persistent exposure to alcohol. Using an olfactory conditioning assay where an odorant is implemented as a conditioned stimulus and paired with a heat shock as an unconditioned stimulus, a previous study has shown that when exposed to a short acute dose of alcohol, Drosophila larvae can no longer learn this association. Interestingly, upon prolonged chronic alcohol exposure, larvae seem to successfully avoid the conditioned stimulus just as well as control alcohol-naive larvae, suggestive of alcohol-induced neuroadaptations. However, the mechanisms by which Drosophila adapt to the presence of alcohol remains unknown. In this study, we explore the transcriptional correlates of neuroadaptation in Drosophila larvae exposed to chronic alcohol to understand the genetic and cellular components responsible for this adaptation. For this, we employed RNA sequencing technology to evaluate differences in gene expression in the brain of larvae chronically exposed to alcohol. Our results suggest that alcohol-induced neuroadaptations are modulated by a diverse array of synaptic genes within the larval brain through a series of epigenetic modulators. Frontiers Media S.A. 2021-11-04 /pmc/articles/PMC8599819/ /pubmed/34803626 http://dx.doi.org/10.3389/fnbeh.2021.768694 Text en Copyright © 2021 Anqueira-González, Acevedo-Gonzalez, Montes-Mercado, Irizarry-Hernández, Fuenzalida-Uribe and Ghezzi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Anqueira-González, Amanda
Acevedo-Gonzalez, Jenny P.
Montes-Mercado, Airined
Irizarry-Hernández, Claudia
Fuenzalida-Uribe, Nicolás L.
Ghezzi, Alfredo
Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_full Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_fullStr Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_full_unstemmed Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_short Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_sort transcriptional correlates of chronic alcohol neuroadaptation in drosophila larvae
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599819/
https://www.ncbi.nlm.nih.gov/pubmed/34803626
http://dx.doi.org/10.3389/fnbeh.2021.768694
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