An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to...

Descripción completa

Detalles Bibliográficos
Autores principales: Milosevic, Jadranka, Lu, Mengrou, Greene, Wallace, He, Hong-Zhang, Zheng, Si-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600229/
https://www.ncbi.nlm.nih.gov/pubmed/34803970
http://dx.doi.org/10.3389/fmicb.2021.749783
_version_ 1784601106683265024
author Milosevic, Jadranka
Lu, Mengrou
Greene, Wallace
He, Hong-Zhang
Zheng, Si-Yang
author_facet Milosevic, Jadranka
Lu, Mengrou
Greene, Wallace
He, Hong-Zhang
Zheng, Si-Yang
author_sort Milosevic, Jadranka
collection PubMed
description We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.
format Online
Article
Text
id pubmed-8600229
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86002292021-11-19 An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 Milosevic, Jadranka Lu, Mengrou Greene, Wallace He, Hong-Zhang Zheng, Si-Yang Front Microbiol Microbiology We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%. Frontiers Media S.A. 2021-11-04 /pmc/articles/PMC8600229/ /pubmed/34803970 http://dx.doi.org/10.3389/fmicb.2021.749783 Text en Copyright © 2021 Milosevic, Lu, Greene, He and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Milosevic, Jadranka
Lu, Mengrou
Greene, Wallace
He, Hong-Zhang
Zheng, Si-Yang
An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_full An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_fullStr An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_full_unstemmed An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_short An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_sort ultrafast one-step quantitative reverse transcription–polymerase chain reaction assay for detection of sars-cov-2
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600229/
https://www.ncbi.nlm.nih.gov/pubmed/34803970
http://dx.doi.org/10.3389/fmicb.2021.749783
work_keys_str_mv AT milosevicjadranka anultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT lumengrou anultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT greenewallace anultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT hehongzhang anultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT zhengsiyang anultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT milosevicjadranka ultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT lumengrou ultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT greenewallace ultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT hehongzhang ultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2
AT zhengsiyang ultrafastonestepquantitativereversetranscriptionpolymerasechainreactionassayfordetectionofsarscov2

Ejemplares similares