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Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more

BACKGROUND: Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiota...

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Autores principales: Giraud, Carolane, Callac, Nolwenn, Beauvais, Maxime, Mailliez, Jean-René, Ansquer, Dominique, Selmaoui-Folcher, Nazha, Pham, Dominique, Wabete, Nelly, Boulo, Viviane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601056/
https://www.ncbi.nlm.nih.gov/pubmed/34820157
http://dx.doi.org/10.7717/peerj.12241
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author Giraud, Carolane
Callac, Nolwenn
Beauvais, Maxime
Mailliez, Jean-René
Ansquer, Dominique
Selmaoui-Folcher, Nazha
Pham, Dominique
Wabete, Nelly
Boulo, Viviane
author_facet Giraud, Carolane
Callac, Nolwenn
Beauvais, Maxime
Mailliez, Jean-René
Ansquer, Dominique
Selmaoui-Folcher, Nazha
Pham, Dominique
Wabete, Nelly
Boulo, Viviane
author_sort Giraud, Carolane
collection PubMed
description BACKGROUND: Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiotas of farmed species and their rearing environment could help establish precise bacterial management. METHOD: In light of these facts, we studied the active microbial communities associated with the eggs and the nauplii of the Pacific blue shrimp (Litopenaeus stylirostris) and their rearing water. All samples were collected in September 2018, November 2018 and February 2019. After RNA extractions, two distinct Illumina HiSeq sequencings were performed. Due to different sequencing depths and in order to compare samples, data were normalized using the Count Per Million method. RESULTS: We found a core microbiota made of taxa related to Aestuariibacter, Alteromonas, Vibrio, SAR11, HIMB11, AEGEAN 169 marine group and Candidatus Endobugula associated with all the samples indicating that these bacterial communities could be transferred from the water to the animals. We also highlighted specific bacterial taxa in the eggs and the nauplii affiliated to Pseudomonas, Corynebacterium, Acinetobacter, Labrenzia, Rothia, Thalassolituus, Marinobacter, Aureispira, Oleiphilus, Profundimonas and Marinobacterium genera suggesting a possible prokaryotic vertical transmission from the breeders to their offspring. This study is the first to focus on the active microbiota associated with early developmental stages of a farmed shrimp species and could serve as a basis to comprehend the microbial interactions involved throughout the whole rearing process.
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spelling pubmed-86010562021-11-23 Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more Giraud, Carolane Callac, Nolwenn Beauvais, Maxime Mailliez, Jean-René Ansquer, Dominique Selmaoui-Folcher, Nazha Pham, Dominique Wabete, Nelly Boulo, Viviane PeerJ Aquaculture, Fisheries and Fish Science BACKGROUND: Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiotas of farmed species and their rearing environment could help establish precise bacterial management. METHOD: In light of these facts, we studied the active microbial communities associated with the eggs and the nauplii of the Pacific blue shrimp (Litopenaeus stylirostris) and their rearing water. All samples were collected in September 2018, November 2018 and February 2019. After RNA extractions, two distinct Illumina HiSeq sequencings were performed. Due to different sequencing depths and in order to compare samples, data were normalized using the Count Per Million method. RESULTS: We found a core microbiota made of taxa related to Aestuariibacter, Alteromonas, Vibrio, SAR11, HIMB11, AEGEAN 169 marine group and Candidatus Endobugula associated with all the samples indicating that these bacterial communities could be transferred from the water to the animals. We also highlighted specific bacterial taxa in the eggs and the nauplii affiliated to Pseudomonas, Corynebacterium, Acinetobacter, Labrenzia, Rothia, Thalassolituus, Marinobacter, Aureispira, Oleiphilus, Profundimonas and Marinobacterium genera suggesting a possible prokaryotic vertical transmission from the breeders to their offspring. This study is the first to focus on the active microbiota associated with early developmental stages of a farmed shrimp species and could serve as a basis to comprehend the microbial interactions involved throughout the whole rearing process. PeerJ Inc. 2021-11-15 /pmc/articles/PMC8601056/ /pubmed/34820157 http://dx.doi.org/10.7717/peerj.12241 Text en ©2021 Giraud et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Giraud, Carolane
Callac, Nolwenn
Beauvais, Maxime
Mailliez, Jean-René
Ansquer, Dominique
Selmaoui-Folcher, Nazha
Pham, Dominique
Wabete, Nelly
Boulo, Viviane
Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
title Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
title_full Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
title_fullStr Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
title_full_unstemmed Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
title_short Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more
title_sort potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp litopenaeus stylirostris: possible influence of the rearing water and more
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601056/
https://www.ncbi.nlm.nih.gov/pubmed/34820157
http://dx.doi.org/10.7717/peerj.12241
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