Cargando…

Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies

Complete genome resequencing was conducted for Thermus thermophilus strain TMY by hybrid assembly of Oxford Nanopore Technologies long-read and MGI short-read data. Errors in the previously reported genome sequence determined by PacBio technology alone were corrected, allowing for high-quality compa...

Descripción completa

Detalles Bibliográficos
Autores principales: Miyazaki, Kentaro, Tokito, Natsuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601142/
https://www.ncbi.nlm.nih.gov/pubmed/34792380
http://dx.doi.org/10.1128/MRA.00979-21
_version_ 1784601282726592512
author Miyazaki, Kentaro
Tokito, Natsuko
author_facet Miyazaki, Kentaro
Tokito, Natsuko
author_sort Miyazaki, Kentaro
collection PubMed
description Complete genome resequencing was conducted for Thermus thermophilus strain TMY by hybrid assembly of Oxford Nanopore Technologies long-read and MGI short-read data. Errors in the previously reported genome sequence determined by PacBio technology alone were corrected, allowing for high-quality comparative genomic analysis of closely related T. thermophilus genomes.
format Online
Article
Text
id pubmed-8601142
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-86011422021-12-07 Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies Miyazaki, Kentaro Tokito, Natsuko Microbiol Resour Announc Genome Sequences Complete genome resequencing was conducted for Thermus thermophilus strain TMY by hybrid assembly of Oxford Nanopore Technologies long-read and MGI short-read data. Errors in the previously reported genome sequence determined by PacBio technology alone were corrected, allowing for high-quality comparative genomic analysis of closely related T. thermophilus genomes. American Society for Microbiology 2021-11-18 /pmc/articles/PMC8601142/ /pubmed/34792380 http://dx.doi.org/10.1128/MRA.00979-21 Text en Copyright © 2021 Miyazaki and Tokito. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Genome Sequences
Miyazaki, Kentaro
Tokito, Natsuko
Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies
title Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies
title_full Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies
title_fullStr Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies
title_full_unstemmed Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies
title_short Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies
title_sort complete genome resequencing of thermus thermophilus strain tmy by hybrid assembly of long- and short-read sequencing technologies
topic Genome Sequences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601142/
https://www.ncbi.nlm.nih.gov/pubmed/34792380
http://dx.doi.org/10.1128/MRA.00979-21
work_keys_str_mv AT miyazakikentaro completegenomeresequencingofthermusthermophilusstraintmybyhybridassemblyoflongandshortreadsequencingtechnologies
AT tokitonatsuko completegenomeresequencingofthermusthermophilusstraintmybyhybridassemblyoflongandshortreadsequencingtechnologies