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LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are guided by transposon mutagenesis studies, coupled to deepsequencing (TnSeq) to identify insertion...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601177/ https://www.ncbi.nlm.nih.gov/pubmed/33325626 http://dx.doi.org/10.1111/1751-7915.13714 |