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Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2 spike Mutations Associated with Variants of Concern

To provide an accessible and inexpensive method to surveil for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations, we developed a multiplex real-time reverse transcription-PCR (rRT-PCR) assay, the Spike single-nucleotide polymorphism (SNP) assay, to detect specific mutations in t...

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Detalles Bibliográficos
Autores principales: Babiker, Ahmed, Immergluck, Katherine, Stampfer, Samuel D., Rao, Anuradha, Bassit, Leda, Su, Max, Nguyen, Vi, Stittleburg, Victoria, Ingersoll, Jessica M., Bradley, Heath L., Mavigner, Maud, Schoof, Nils, Kraft, Colleen S., Chahroudi, Ann, Schinazi, Raymond F., Martin, Greg S., Piantadosi, Anne, Lam, Wilbur A., Waggoner, Jesse J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601233/
https://www.ncbi.nlm.nih.gov/pubmed/34432488
http://dx.doi.org/10.1128/JCM.01446-21

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