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Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2 spike Mutations Associated with Variants of Concern
To provide an accessible and inexpensive method to surveil for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations, we developed a multiplex real-time reverse transcription-PCR (rRT-PCR) assay, the Spike single-nucleotide polymorphism (SNP) assay, to detect specific mutations in t...
Autores principales: | Babiker, Ahmed, Immergluck, Katherine, Stampfer, Samuel D., Rao, Anuradha, Bassit, Leda, Su, Max, Nguyen, Vi, Stittleburg, Victoria, Ingersoll, Jessica M., Bradley, Heath L., Mavigner, Maud, Schoof, Nils, Kraft, Colleen S., Chahroudi, Ann, Schinazi, Raymond F., Martin, Greg S., Piantadosi, Anne, Lam, Wilbur A., Waggoner, Jesse J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601233/ https://www.ncbi.nlm.nih.gov/pubmed/34432488 http://dx.doi.org/10.1128/JCM.01446-21 |
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