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The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study

The inhibitor of apoptosis family proteins (IAPs) plays a crucial role in the process of carcinogenesis by regulating apoptosis and maintaining the tissue balance. In this study, a transcriptomic analysis of IAP-encoding genes in colon cancer was performed using oligonucleotide microarrays. Adenocar...

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Autores principales: Waniczek, Dariusz, Nowak, Marcin, Lorenc-Góra, Justyna, Muc-Wierzgoń, Małgorzata, Mazurek, Urszula, Bichalska-Lach, Magda, Lorenc, Zbigniew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601263/
https://www.ncbi.nlm.nih.gov/pubmed/34797333
http://dx.doi.org/10.1097/MD.0000000000027882
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author Waniczek, Dariusz
Nowak, Marcin
Lorenc-Góra, Justyna
Muc-Wierzgoń, Małgorzata
Mazurek, Urszula
Bichalska-Lach, Magda
Lorenc, Zbigniew
author_facet Waniczek, Dariusz
Nowak, Marcin
Lorenc-Góra, Justyna
Muc-Wierzgoń, Małgorzata
Mazurek, Urszula
Bichalska-Lach, Magda
Lorenc, Zbigniew
author_sort Waniczek, Dariusz
collection PubMed
description The inhibitor of apoptosis family proteins (IAPs) plays a crucial role in the process of carcinogenesis by regulating apoptosis and maintaining the tissue balance. In this study, a transcriptomic analysis of IAP-encoding genes in colon cancer was performed using oligonucleotide microarrays. Adenocarcinoma and healthy colon tissue samples were collected from 32 patients (16 females and 16 males) who underwent surgery due to colon cancer. The mRNA was extracted from tissue samples and tested using oligonucleotide microarrays (Affymetrix). The results were validated using the qRT-PCR technique. Hierarchical grouping was used to allocate 37 samples of normalized mRNA concentrations into 4 groups, with statistically significant differences in gene expression between these groups. The group of genes associated with colon cancer, including IAP-encoding gene - BIRC5 (Survivin), was selected for further testing. Our study confirmed an increased expression of BIRC5 in colon cancer tissue when compared to the control group. Increased levels of Neuronal Apoptosis Inhibitory Proteins were detected only in low-stage colon cancer, while the expression of Human X Chromosome-Encoded inhibitor of apoptosis family proteins decreased in colon cancer. The transcriptional activity of IAP-encoding genes varied, depending on the severity of colon cancer. The concentration of mRNA, encoding BIRC5 was elevated in samples obtained from more advanced colon cancer. Hence BIRC5 could be used as a complementary parameter for the diagnosis and prognosis of colon cancer.
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spelling pubmed-86012632021-11-20 The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study Waniczek, Dariusz Nowak, Marcin Lorenc-Góra, Justyna Muc-Wierzgoń, Małgorzata Mazurek, Urszula Bichalska-Lach, Magda Lorenc, Zbigniew Medicine (Baltimore) 5700 The inhibitor of apoptosis family proteins (IAPs) plays a crucial role in the process of carcinogenesis by regulating apoptosis and maintaining the tissue balance. In this study, a transcriptomic analysis of IAP-encoding genes in colon cancer was performed using oligonucleotide microarrays. Adenocarcinoma and healthy colon tissue samples were collected from 32 patients (16 females and 16 males) who underwent surgery due to colon cancer. The mRNA was extracted from tissue samples and tested using oligonucleotide microarrays (Affymetrix). The results were validated using the qRT-PCR technique. Hierarchical grouping was used to allocate 37 samples of normalized mRNA concentrations into 4 groups, with statistically significant differences in gene expression between these groups. The group of genes associated with colon cancer, including IAP-encoding gene - BIRC5 (Survivin), was selected for further testing. Our study confirmed an increased expression of BIRC5 in colon cancer tissue when compared to the control group. Increased levels of Neuronal Apoptosis Inhibitory Proteins were detected only in low-stage colon cancer, while the expression of Human X Chromosome-Encoded inhibitor of apoptosis family proteins decreased in colon cancer. The transcriptional activity of IAP-encoding genes varied, depending on the severity of colon cancer. The concentration of mRNA, encoding BIRC5 was elevated in samples obtained from more advanced colon cancer. Hence BIRC5 could be used as a complementary parameter for the diagnosis and prognosis of colon cancer. Lippincott Williams & Wilkins 2021-11-19 /pmc/articles/PMC8601263/ /pubmed/34797333 http://dx.doi.org/10.1097/MD.0000000000027882 Text en Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle 5700
Waniczek, Dariusz
Nowak, Marcin
Lorenc-Góra, Justyna
Muc-Wierzgoń, Małgorzata
Mazurek, Urszula
Bichalska-Lach, Magda
Lorenc, Zbigniew
The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study
title The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study
title_full The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study
title_fullStr The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study
title_full_unstemmed The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study
title_short The transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: A STROBE-compliant study
title_sort transcriptional activity profile of inhibitor apoptosis protein encoding genes in colon cancer patients: a strobe-compliant study
topic 5700
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601263/
https://www.ncbi.nlm.nih.gov/pubmed/34797333
http://dx.doi.org/10.1097/MD.0000000000027882
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