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Electronic control of redox reactions inside Escherichia coli using a genetic module

Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we...

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Detalles Bibliográficos
Autores principales: Baruch, Moshe, Tejedor-Sanz, Sara, Su, Lin, Ajo-Franklin, Caroline M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601525/
https://www.ncbi.nlm.nih.gov/pubmed/34793478
http://dx.doi.org/10.1371/journal.pone.0258380
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author Baruch, Moshe
Tejedor-Sanz, Sara
Su, Lin
Ajo-Franklin, Caroline M.
author_facet Baruch, Moshe
Tejedor-Sanz, Sara
Su, Lin
Ajo-Franklin, Caroline M.
author_sort Baruch, Moshe
collection PubMed
description Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.
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spelling pubmed-86015252021-11-19 Electronic control of redox reactions inside Escherichia coli using a genetic module Baruch, Moshe Tejedor-Sanz, Sara Su, Lin Ajo-Franklin, Caroline M. PLoS One Research Article Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism. Public Library of Science 2021-11-18 /pmc/articles/PMC8601525/ /pubmed/34793478 http://dx.doi.org/10.1371/journal.pone.0258380 Text en © 2021 Baruch et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Baruch, Moshe
Tejedor-Sanz, Sara
Su, Lin
Ajo-Franklin, Caroline M.
Electronic control of redox reactions inside Escherichia coli using a genetic module
title Electronic control of redox reactions inside Escherichia coli using a genetic module
title_full Electronic control of redox reactions inside Escherichia coli using a genetic module
title_fullStr Electronic control of redox reactions inside Escherichia coli using a genetic module
title_full_unstemmed Electronic control of redox reactions inside Escherichia coli using a genetic module
title_short Electronic control of redox reactions inside Escherichia coli using a genetic module
title_sort electronic control of redox reactions inside escherichia coli using a genetic module
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601525/
https://www.ncbi.nlm.nih.gov/pubmed/34793478
http://dx.doi.org/10.1371/journal.pone.0258380
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