Cargando…
Electronic control of redox reactions inside Escherichia coli using a genetic module
Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601525/ https://www.ncbi.nlm.nih.gov/pubmed/34793478 http://dx.doi.org/10.1371/journal.pone.0258380 |
_version_ | 1784601370622427136 |
---|---|
author | Baruch, Moshe Tejedor-Sanz, Sara Su, Lin Ajo-Franklin, Caroline M. |
author_facet | Baruch, Moshe Tejedor-Sanz, Sara Su, Lin Ajo-Franklin, Caroline M. |
author_sort | Baruch, Moshe |
collection | PubMed |
description | Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism. |
format | Online Article Text |
id | pubmed-8601525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-86015252021-11-19 Electronic control of redox reactions inside Escherichia coli using a genetic module Baruch, Moshe Tejedor-Sanz, Sara Su, Lin Ajo-Franklin, Caroline M. PLoS One Research Article Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism. Public Library of Science 2021-11-18 /pmc/articles/PMC8601525/ /pubmed/34793478 http://dx.doi.org/10.1371/journal.pone.0258380 Text en © 2021 Baruch et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Baruch, Moshe Tejedor-Sanz, Sara Su, Lin Ajo-Franklin, Caroline M. Electronic control of redox reactions inside Escherichia coli using a genetic module |
title | Electronic control of redox reactions inside Escherichia coli using a genetic module |
title_full | Electronic control of redox reactions inside Escherichia coli using a genetic module |
title_fullStr | Electronic control of redox reactions inside Escherichia coli using a genetic module |
title_full_unstemmed | Electronic control of redox reactions inside Escherichia coli using a genetic module |
title_short | Electronic control of redox reactions inside Escherichia coli using a genetic module |
title_sort | electronic control of redox reactions inside escherichia coli using a genetic module |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601525/ https://www.ncbi.nlm.nih.gov/pubmed/34793478 http://dx.doi.org/10.1371/journal.pone.0258380 |
work_keys_str_mv | AT baruchmoshe electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule AT tejedorsanzsara electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule AT sulin electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule AT ajofranklincarolinem electroniccontrolofredoxreactionsinsideescherichiacoliusingageneticmodule |