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Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway

Retinoblastoma (RB) is one of the most common intraocular malignancies in children, which causes vision loss and even threatens life. Eriodictyol is a natural flavonoid with strong anticancer activity. Some studies have shown that eriodictyol exerts anticancer effects in glioma, colon cancer, and lu...

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Detalles Bibliográficos
Autores principales: Wen, Shu, Hu, Meng, Xiong, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601792/
https://www.ncbi.nlm.nih.gov/pubmed/34804455
http://dx.doi.org/10.1155/2021/6091585
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author Wen, Shu
Hu, Meng
Xiong, Yan
author_facet Wen, Shu
Hu, Meng
Xiong, Yan
author_sort Wen, Shu
collection PubMed
description Retinoblastoma (RB) is one of the most common intraocular malignancies in children, which causes vision loss and even threatens life. Eriodictyol is a natural flavonoid with strong anticancer activity. Some studies have shown that eriodictyol exerts anticancer effects in glioma, colon cancer, and lung cancer; however, no studies have reported the anticancer effects of eriodictyol on RB. Therefore, the aim of this study was to investigate the anticancer activity of eriodictyol against the RB Y79 cell line and its potential mechanism of action. Interestingly, we found that eriodictyol inhibited the proliferation, migration, and invasion of Y79 cells in a dose-dependent manner and decreased the expression of MMP-2 and MMP-9 proteins in the cells. In addition, eriodictyol-induced apoptosis in Y79 cells was assessed by flow cytometry and immunoblotting. Here, our study revealed that eriodictyol dose dependently inhibited the activation of the PI3K/Akt signaling pathway. Notably, the effect of eriodictyol on RB apoptosis was reversed by a PI3K agonist 740 Y-P. In conclusion, our study shows that eriodictyol effectively inhibits proliferation, migration, and invasion and induces apoptosis in RB cell lines, which may be the result of blocking the PI3K/Akt signaling pathway. Thus, eriodictyol may provide a new theoretical basis for exploring targeted antitumor natural therapies.
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spelling pubmed-86017922021-11-19 Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway Wen, Shu Hu, Meng Xiong, Yan J Healthc Eng Research Article Retinoblastoma (RB) is one of the most common intraocular malignancies in children, which causes vision loss and even threatens life. Eriodictyol is a natural flavonoid with strong anticancer activity. Some studies have shown that eriodictyol exerts anticancer effects in glioma, colon cancer, and lung cancer; however, no studies have reported the anticancer effects of eriodictyol on RB. Therefore, the aim of this study was to investigate the anticancer activity of eriodictyol against the RB Y79 cell line and its potential mechanism of action. Interestingly, we found that eriodictyol inhibited the proliferation, migration, and invasion of Y79 cells in a dose-dependent manner and decreased the expression of MMP-2 and MMP-9 proteins in the cells. In addition, eriodictyol-induced apoptosis in Y79 cells was assessed by flow cytometry and immunoblotting. Here, our study revealed that eriodictyol dose dependently inhibited the activation of the PI3K/Akt signaling pathway. Notably, the effect of eriodictyol on RB apoptosis was reversed by a PI3K agonist 740 Y-P. In conclusion, our study shows that eriodictyol effectively inhibits proliferation, migration, and invasion and induces apoptosis in RB cell lines, which may be the result of blocking the PI3K/Akt signaling pathway. Thus, eriodictyol may provide a new theoretical basis for exploring targeted antitumor natural therapies. Hindawi 2021-11-11 /pmc/articles/PMC8601792/ /pubmed/34804455 http://dx.doi.org/10.1155/2021/6091585 Text en Copyright © 2021 Shu Wen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wen, Shu
Hu, Meng
Xiong, Yan
Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway
title Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway
title_full Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway
title_fullStr Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway
title_full_unstemmed Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway
title_short Effect of Eriodictyol on Retinoblastoma via the PI3K/Akt Pathway
title_sort effect of eriodictyol on retinoblastoma via the pi3k/akt pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601792/
https://www.ncbi.nlm.nih.gov/pubmed/34804455
http://dx.doi.org/10.1155/2021/6091585
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