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A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes

Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut mic...

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Autores principales: Darnaud, Marion, De Vadder, Filipe, Bogeat, Pascaline, Boucinha, Lilia, Bulteau, Anne-Laure, Bunescu, Andrei, Couturier, Céline, Delgado, Ana, Dugua, Hélène, Elie, Céline, Mathieu, Alban, Novotná, Tereza, Ouattara, Djomangan Adama, Planel, Séverine, Saliou, Adrien, Šrůtková, Dagmar, Yansouni, Jennifer, Stecher, Bärbel, Schwarzer, Martin, Leulier, François, Tamellini, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602333/
https://www.ncbi.nlm.nih.gov/pubmed/34795236
http://dx.doi.org/10.1038/s41467-021-26963-9
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author Darnaud, Marion
De Vadder, Filipe
Bogeat, Pascaline
Boucinha, Lilia
Bulteau, Anne-Laure
Bunescu, Andrei
Couturier, Céline
Delgado, Ana
Dugua, Hélène
Elie, Céline
Mathieu, Alban
Novotná, Tereza
Ouattara, Djomangan Adama
Planel, Séverine
Saliou, Adrien
Šrůtková, Dagmar
Yansouni, Jennifer
Stecher, Bärbel
Schwarzer, Martin
Leulier, François
Tamellini, Andrea
author_facet Darnaud, Marion
De Vadder, Filipe
Bogeat, Pascaline
Boucinha, Lilia
Bulteau, Anne-Laure
Bunescu, Andrei
Couturier, Céline
Delgado, Ana
Dugua, Hélène
Elie, Céline
Mathieu, Alban
Novotná, Tereza
Ouattara, Djomangan Adama
Planel, Séverine
Saliou, Adrien
Šrůtková, Dagmar
Yansouni, Jennifer
Stecher, Bärbel
Schwarzer, Martin
Leulier, François
Tamellini, Andrea
author_sort Darnaud, Marion
collection PubMed
description Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut microbiome in preclinical mouse studies, here we report the development of a simplified mouse microbiota composed of 15 strains from 7 of the 20 most prevalent bacterial families representative of the fecal microbiota of C57BL/6J Specific (and Opportunistic) Pathogen-Free (SPF/SOPF) animals and the derivation of a standardized gnotobiotic mouse model called GM15. GM15 recapitulates extensively the functionalities found in the C57BL/6J SOPF microbiota metagenome, and GM15 animals are phenotypically similar to SOPF or SPF animals in two different facilities. They are also less sensitive to the deleterious effects of post-weaning malnutrition. In this work, we show that the GM15 model provides increased reproducibility and robustness of preclinical studies by limiting the confounding effect of fluctuation in microbiota composition, and offers opportunities for research focused on how the microbiota shapes host physiology in health and disease.
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spelling pubmed-86023332021-11-19 A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes Darnaud, Marion De Vadder, Filipe Bogeat, Pascaline Boucinha, Lilia Bulteau, Anne-Laure Bunescu, Andrei Couturier, Céline Delgado, Ana Dugua, Hélène Elie, Céline Mathieu, Alban Novotná, Tereza Ouattara, Djomangan Adama Planel, Séverine Saliou, Adrien Šrůtková, Dagmar Yansouni, Jennifer Stecher, Bärbel Schwarzer, Martin Leulier, François Tamellini, Andrea Nat Commun Article Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut microbiome in preclinical mouse studies, here we report the development of a simplified mouse microbiota composed of 15 strains from 7 of the 20 most prevalent bacterial families representative of the fecal microbiota of C57BL/6J Specific (and Opportunistic) Pathogen-Free (SPF/SOPF) animals and the derivation of a standardized gnotobiotic mouse model called GM15. GM15 recapitulates extensively the functionalities found in the C57BL/6J SOPF microbiota metagenome, and GM15 animals are phenotypically similar to SOPF or SPF animals in two different facilities. They are also less sensitive to the deleterious effects of post-weaning malnutrition. In this work, we show that the GM15 model provides increased reproducibility and robustness of preclinical studies by limiting the confounding effect of fluctuation in microbiota composition, and offers opportunities for research focused on how the microbiota shapes host physiology in health and disease. Nature Publishing Group UK 2021-11-18 /pmc/articles/PMC8602333/ /pubmed/34795236 http://dx.doi.org/10.1038/s41467-021-26963-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Darnaud, Marion
De Vadder, Filipe
Bogeat, Pascaline
Boucinha, Lilia
Bulteau, Anne-Laure
Bunescu, Andrei
Couturier, Céline
Delgado, Ana
Dugua, Hélène
Elie, Céline
Mathieu, Alban
Novotná, Tereza
Ouattara, Djomangan Adama
Planel, Séverine
Saliou, Adrien
Šrůtková, Dagmar
Yansouni, Jennifer
Stecher, Bärbel
Schwarzer, Martin
Leulier, François
Tamellini, Andrea
A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
title A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
title_full A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
title_fullStr A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
title_full_unstemmed A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
title_short A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
title_sort standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates sopf/spf phenotypes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602333/
https://www.ncbi.nlm.nih.gov/pubmed/34795236
http://dx.doi.org/10.1038/s41467-021-26963-9
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