Cargando…

Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions

Salmonella enterica strains often harbor plasmids representing several incompatibility groups (Inc) including IncFIB, which have been previously associated with carrying antimicrobial resistance and virulence associated genes. To better understand the distribution of virulence genes on IncFIB plasmi...

Descripción completa

Detalles Bibliográficos
Autores principales: Abbott, Carter N., Felix, Monique, Foley, Steven L., Khajanchi, Bijay K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602916/
https://www.ncbi.nlm.nih.gov/pubmed/34803945
http://dx.doi.org/10.3389/fmicb.2021.729275
_version_ 1784601665099268096
author Abbott, Carter N.
Felix, Monique
Foley, Steven L.
Khajanchi, Bijay K.
author_facet Abbott, Carter N.
Felix, Monique
Foley, Steven L.
Khajanchi, Bijay K.
author_sort Abbott, Carter N.
collection PubMed
description Salmonella enterica strains often harbor plasmids representing several incompatibility groups (Inc) including IncFIB, which have been previously associated with carrying antimicrobial resistance and virulence associated genes. To better understand the distribution of virulence genes on IncFIB plasmids, we analyzed 37 complete whole genome and plasmid sequences of different S. enterica isolates from multiple serovars. Many of the sequences analyzed carried multiple virulence-associated genes, including those associated with iron acquisition systems; thus we aimed to determine how iron-rich (IR) and various iron-depleted (ID) conditions affected the transcription of iron acquisition and virulence genes including sitA, iutA, iucA, and enolase at different time intervals. sitA, iutA, and enolase from S. enterica that were grown in Luria-Bertani broth (LB) ID (LBID) conditions were substantially upregulated when compared to LBIR conditions. For both S. enterica strains that were grown at various LBID conditions, addition of 200 μM bipyridyl in the growth medium yielded the highest transcription for all four genes, followed by the 100 μM concentration. An antibody using a peptide targeting aerobactin receptor gene iutA encoded by IncFIB was generated and used to examine the protein expression in the wild-type, recipient, and transconjugant strain in LB, LBID, and LBIR growth conditions using Western blot analyses. A 70 KDa protein band was detected in the wild-type and transconjugant that carried the IncFIB plasmid, while this band was not detected in the recipient strain that lacked this plasmid.
format Online
Article
Text
id pubmed-8602916
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86029162021-11-20 Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions Abbott, Carter N. Felix, Monique Foley, Steven L. Khajanchi, Bijay K. Front Microbiol Microbiology Salmonella enterica strains often harbor plasmids representing several incompatibility groups (Inc) including IncFIB, which have been previously associated with carrying antimicrobial resistance and virulence associated genes. To better understand the distribution of virulence genes on IncFIB plasmids, we analyzed 37 complete whole genome and plasmid sequences of different S. enterica isolates from multiple serovars. Many of the sequences analyzed carried multiple virulence-associated genes, including those associated with iron acquisition systems; thus we aimed to determine how iron-rich (IR) and various iron-depleted (ID) conditions affected the transcription of iron acquisition and virulence genes including sitA, iutA, iucA, and enolase at different time intervals. sitA, iutA, and enolase from S. enterica that were grown in Luria-Bertani broth (LB) ID (LBID) conditions were substantially upregulated when compared to LBIR conditions. For both S. enterica strains that were grown at various LBID conditions, addition of 200 μM bipyridyl in the growth medium yielded the highest transcription for all four genes, followed by the 100 μM concentration. An antibody using a peptide targeting aerobactin receptor gene iutA encoded by IncFIB was generated and used to examine the protein expression in the wild-type, recipient, and transconjugant strain in LB, LBID, and LBIR growth conditions using Western blot analyses. A 70 KDa protein band was detected in the wild-type and transconjugant that carried the IncFIB plasmid, while this band was not detected in the recipient strain that lacked this plasmid. Frontiers Media S.A. 2021-11-05 /pmc/articles/PMC8602916/ /pubmed/34803945 http://dx.doi.org/10.3389/fmicb.2021.729275 Text en Copyright © 2021 Abbott, Felix, Foley and Khajanchi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Abbott, Carter N.
Felix, Monique
Foley, Steven L.
Khajanchi, Bijay K.
Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_full Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_fullStr Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_full_unstemmed Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_short Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions
title_sort expression of genes located on the incompatibility group fib plasmids at transcription and protein levels in iron-modified growth conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602916/
https://www.ncbi.nlm.nih.gov/pubmed/34803945
http://dx.doi.org/10.3389/fmicb.2021.729275
work_keys_str_mv AT abbottcartern expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions
AT felixmonique expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions
AT foleystevenl expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions
AT khajanchibijayk expressionofgeneslocatedontheincompatibilitygroupfibplasmidsattranscriptionandproteinlevelsinironmodifiedgrowthconditions