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FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice
The acrosome is a cap-shaped, Golgi-derived membranous organelle that is located over the anterior of the sperm nucleus and highly conserved throughout evolution. Although morphological changes during acrosome biogenesis in spermatogenesis have been well described, the molecular mechanism underlying...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602946/ https://www.ncbi.nlm.nih.gov/pubmed/34714330 http://dx.doi.org/10.1242/dev.199644 |
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author | Morohoshi, Akane Miyata, Haruhiko Oyama, Yuki Oura, Seiya Noda, Taichi Ikawa, Masahito |
author_facet | Morohoshi, Akane Miyata, Haruhiko Oyama, Yuki Oura, Seiya Noda, Taichi Ikawa, Masahito |
author_sort | Morohoshi, Akane |
collection | PubMed |
description | The acrosome is a cap-shaped, Golgi-derived membranous organelle that is located over the anterior of the sperm nucleus and highly conserved throughout evolution. Although morphological changes during acrosome biogenesis in spermatogenesis have been well described, the molecular mechanism underlying this process is still largely unknown. Family with sequence similarity 71, member F1 and F2 (FAM71F1 and FAM71F2) are testis-enriched proteins that contain a RAB2B-binding domain, a small GTPase involved in vesicle transport and membrane trafficking. Here, by generating mutant mice for each gene, we found that Fam71f1 is essential for male fertility. In Fam71f1-mutant mice, the acrosome was abnormally expanded at the round spermatid stage, likely because of enhanced vesicle trafficking. Mass spectrometry analysis after immunoprecipitation indicated that, in testes, FAM71F1 binds not only RAB2B, but also RAB2A. Further study suggested that FAM71F1 binds to the GTP-bound active form of RAB2A/B, but not the inactive form. These results indicate that a complex of FAM71F1 and active RAB2A/B suppresses excessive vesicle trafficking during acrosome formation. |
format | Online Article Text |
id | pubmed-8602946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-86029462021-11-30 FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice Morohoshi, Akane Miyata, Haruhiko Oyama, Yuki Oura, Seiya Noda, Taichi Ikawa, Masahito Development Research Article The acrosome is a cap-shaped, Golgi-derived membranous organelle that is located over the anterior of the sperm nucleus and highly conserved throughout evolution. Although morphological changes during acrosome biogenesis in spermatogenesis have been well described, the molecular mechanism underlying this process is still largely unknown. Family with sequence similarity 71, member F1 and F2 (FAM71F1 and FAM71F2) are testis-enriched proteins that contain a RAB2B-binding domain, a small GTPase involved in vesicle transport and membrane trafficking. Here, by generating mutant mice for each gene, we found that Fam71f1 is essential for male fertility. In Fam71f1-mutant mice, the acrosome was abnormally expanded at the round spermatid stage, likely because of enhanced vesicle trafficking. Mass spectrometry analysis after immunoprecipitation indicated that, in testes, FAM71F1 binds not only RAB2B, but also RAB2A. Further study suggested that FAM71F1 binds to the GTP-bound active form of RAB2A/B, but not the inactive form. These results indicate that a complex of FAM71F1 and active RAB2A/B suppresses excessive vesicle trafficking during acrosome formation. The Company of Biologists Ltd 2021-10-29 /pmc/articles/PMC8602946/ /pubmed/34714330 http://dx.doi.org/10.1242/dev.199644 Text en © 2021. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Morohoshi, Akane Miyata, Haruhiko Oyama, Yuki Oura, Seiya Noda, Taichi Ikawa, Masahito FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice |
title | FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice |
title_full | FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice |
title_fullStr | FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice |
title_full_unstemmed | FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice |
title_short | FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice |
title_sort | fam71f1 binds to rab2a and rab2b and is essential for acrosome formation and male fertility in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602946/ https://www.ncbi.nlm.nih.gov/pubmed/34714330 http://dx.doi.org/10.1242/dev.199644 |
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