A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies

The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection....

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Autores principales: Russell, Gabriel A., Peng, Garrie, Faubert, Cynthia, Verdu, Elena F., Hapfelmeier, Siegfried, King, Irah L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603306/
https://www.ncbi.nlm.nih.gov/pubmed/34825215
http://dx.doi.org/10.1016/j.xpro.2021.100946
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author Russell, Gabriel A.
Peng, Garrie
Faubert, Cynthia
Verdu, Elena F.
Hapfelmeier, Siegfried
King, Irah L.
author_facet Russell, Gabriel A.
Peng, Garrie
Faubert, Cynthia
Verdu, Elena F.
Hapfelmeier, Siegfried
King, Irah L.
author_sort Russell, Gabriel A.
collection PubMed
description The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
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spelling pubmed-86033062021-11-24 A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies Russell, Gabriel A. Peng, Garrie Faubert, Cynthia Verdu, Elena F. Hapfelmeier, Siegfried King, Irah L. STAR Protoc Protocol The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions. Elsevier 2021-11-10 /pmc/articles/PMC8603306/ /pubmed/34825215 http://dx.doi.org/10.1016/j.xpro.2021.100946 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Russell, Gabriel A.
Peng, Garrie
Faubert, Cynthia
Verdu, Elena F.
Hapfelmeier, Siegfried
King, Irah L.
A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_full A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_fullStr A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_full_unstemmed A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_short A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
title_sort protocol for generating germ-free heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603306/
https://www.ncbi.nlm.nih.gov/pubmed/34825215
http://dx.doi.org/10.1016/j.xpro.2021.100946
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