Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers

Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation...

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Detalles Bibliográficos
Autores principales: Amberg, Nicole, Hippenmeyer, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603308/
https://www.ncbi.nlm.nih.gov/pubmed/34825212
http://dx.doi.org/10.1016/j.xpro.2021.100939
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author Amberg, Nicole
Hippenmeyer, Simon
author_facet Amberg, Nicole
Hippenmeyer, Simon
author_sort Amberg, Nicole
collection PubMed
description Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).
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spelling pubmed-86033082021-11-24 Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers Amberg, Nicole Hippenmeyer, Simon STAR Protoc Protocol Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021). Elsevier 2021-11-10 /pmc/articles/PMC8603308/ /pubmed/34825212 http://dx.doi.org/10.1016/j.xpro.2021.100939 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Amberg, Nicole
Hippenmeyer, Simon
Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_full Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_fullStr Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_full_unstemmed Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_short Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
title_sort genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603308/
https://www.ncbi.nlm.nih.gov/pubmed/34825212
http://dx.doi.org/10.1016/j.xpro.2021.100939
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