Cargando…

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

BACKGROUND: Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as ins...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Yu, Liu, Chao, Wang, Ming, Wang, Cheng, Yan, Yi-Guo, Wang, Wen-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603486/
https://www.ncbi.nlm.nih.gov/pubmed/34794408
http://dx.doi.org/10.1186/s12891-021-04822-4
_version_ 1784601774682800128
author Kang, Yu
Liu, Chao
Wang, Ming
Wang, Cheng
Yan, Yi-Guo
Wang, Wen-Jun
author_facet Kang, Yu
Liu, Chao
Wang, Ming
Wang, Cheng
Yan, Yi-Guo
Wang, Wen-Jun
author_sort Kang, Yu
collection PubMed
description BACKGROUND: Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery. METHODS: The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and 8 weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis. RESULTS: Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P < 0.05). The BV/TV (B: 42.20 ± 10.50 vs. A: 29.02 ± 3.25, P < 0.05) and Tb.N (B: 4.66 ± 1.23 vs. A: 1.97 ± 0.40, P < 0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10 ± 0.04 vs. A: 0.15 ± 0.02, P < 0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72 ± 12.80 vs. A: 12.36 ± 16.93, P < 0.05). CONCLUSION: A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion.
format Online
Article
Text
id pubmed-8603486
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-86034862021-11-19 A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF) Kang, Yu Liu, Chao Wang, Ming Wang, Cheng Yan, Yi-Guo Wang, Wen-Jun BMC Musculoskelet Disord Research BACKGROUND: Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery. METHODS: The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and 8 weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis. RESULTS: Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P < 0.05). The BV/TV (B: 42.20 ± 10.50 vs. A: 29.02 ± 3.25, P < 0.05) and Tb.N (B: 4.66 ± 1.23 vs. A: 1.97 ± 0.40, P < 0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10 ± 0.04 vs. A: 0.15 ± 0.02, P < 0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72 ± 12.80 vs. A: 12.36 ± 16.93, P < 0.05). CONCLUSION: A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion. BioMed Central 2021-11-18 /pmc/articles/PMC8603486/ /pubmed/34794408 http://dx.doi.org/10.1186/s12891-021-04822-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kang, Yu
Liu, Chao
Wang, Ming
Wang, Cheng
Yan, Yi-Guo
Wang, Wen-Jun
A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_full A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_fullStr A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_full_unstemmed A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_short A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_sort novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (alcf)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603486/
https://www.ncbi.nlm.nih.gov/pubmed/34794408
http://dx.doi.org/10.1186/s12891-021-04822-4
work_keys_str_mv AT kangyu anovelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT liuchao anovelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT wangming anovelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT wangcheng anovelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT yanyiguo anovelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT wangwenjun anovelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT kangyu novelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT liuchao novelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT wangming novelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT wangcheng novelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT yanyiguo novelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf
AT wangwenjun novelratmodelofinterbodyfusionbasedonanteriorlumbarcorpectomyandfusionalcf