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The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
BACKGROUND: The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. M...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604581/ https://www.ncbi.nlm.nih.gov/pubmed/34804181 http://dx.doi.org/10.1155/2021/6065168 |
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author | Lee, Shou-Lun Hsu, Wei-Hsiang Tu, Chia-Ming Wang, Wen-Han Yang, Cheng-Yao Lee, Hsien-Kuang Chin, Ting-Yu |
author_facet | Lee, Shou-Lun Hsu, Wei-Hsiang Tu, Chia-Ming Wang, Wen-Han Yang, Cheng-Yao Lee, Hsien-Kuang Chin, Ting-Yu |
author_sort | Lee, Shou-Lun |
collection | PubMed |
description | BACKGROUND: The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. METHODS: The aHSCs used in this study were derived from hepatic stellate cells that were isolated and purified from the livers of male Wistar rats and then transformed into the activated phenotype by culturing on uncoated plastic dishes. Freshwater clam extract (CE) was collected after the outflow from the live freshwater clams in a water bath at 100°C for 60 min. The effects of CE on aHSCs were analyzed by MTT assay, flow cytometry, Oil Red O (ORO) staining, western blot, and real-time RT-PCR. RESULTS: The results indicated that CE suppressed the proliferation of aHSCs through G0/G1 cell cycle arrest by downregulating cyclin D1 and upregulating p27. The expression levels of a-SMA, collagen I, TGF-β, and TNF-α were inhibited in the CE-treated aHSCs. In addition, the CE treatment increased the lipid contents in aHSCs by promoting PPARγ expression. Furthermore, CE modulated the expression of ECM-related genes, i.e., by upregulating MMP-9 and downregulating TIMP-II. CONCLUSIONS: These data revealed that CE could induce the deactivation of aHSCs. We therefore suggest that CE has potential as an adjuvant therapeutic agent against hepatic fibrosis. |
format | Online Article Text |
id | pubmed-8604581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-86045812021-11-20 The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells Lee, Shou-Lun Hsu, Wei-Hsiang Tu, Chia-Ming Wang, Wen-Han Yang, Cheng-Yao Lee, Hsien-Kuang Chin, Ting-Yu Evid Based Complement Alternat Med Research Article BACKGROUND: The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. METHODS: The aHSCs used in this study were derived from hepatic stellate cells that were isolated and purified from the livers of male Wistar rats and then transformed into the activated phenotype by culturing on uncoated plastic dishes. Freshwater clam extract (CE) was collected after the outflow from the live freshwater clams in a water bath at 100°C for 60 min. The effects of CE on aHSCs were analyzed by MTT assay, flow cytometry, Oil Red O (ORO) staining, western blot, and real-time RT-PCR. RESULTS: The results indicated that CE suppressed the proliferation of aHSCs through G0/G1 cell cycle arrest by downregulating cyclin D1 and upregulating p27. The expression levels of a-SMA, collagen I, TGF-β, and TNF-α were inhibited in the CE-treated aHSCs. In addition, the CE treatment increased the lipid contents in aHSCs by promoting PPARγ expression. Furthermore, CE modulated the expression of ECM-related genes, i.e., by upregulating MMP-9 and downregulating TIMP-II. CONCLUSIONS: These data revealed that CE could induce the deactivation of aHSCs. We therefore suggest that CE has potential as an adjuvant therapeutic agent against hepatic fibrosis. Hindawi 2021-11-12 /pmc/articles/PMC8604581/ /pubmed/34804181 http://dx.doi.org/10.1155/2021/6065168 Text en Copyright © 2021 Shou-Lun Lee et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lee, Shou-Lun Hsu, Wei-Hsiang Tu, Chia-Ming Wang, Wen-Han Yang, Cheng-Yao Lee, Hsien-Kuang Chin, Ting-Yu The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells |
title | The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells |
title_full | The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells |
title_fullStr | The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells |
title_full_unstemmed | The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells |
title_short | The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells |
title_sort | effects of freshwater clam (corbicula fluminea) extract on activated hepatic stellate cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604581/ https://www.ncbi.nlm.nih.gov/pubmed/34804181 http://dx.doi.org/10.1155/2021/6065168 |
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