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The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells

BACKGROUND: The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. M...

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Autores principales: Lee, Shou-Lun, Hsu, Wei-Hsiang, Tu, Chia-Ming, Wang, Wen-Han, Yang, Cheng-Yao, Lee, Hsien-Kuang, Chin, Ting-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604581/
https://www.ncbi.nlm.nih.gov/pubmed/34804181
http://dx.doi.org/10.1155/2021/6065168
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author Lee, Shou-Lun
Hsu, Wei-Hsiang
Tu, Chia-Ming
Wang, Wen-Han
Yang, Cheng-Yao
Lee, Hsien-Kuang
Chin, Ting-Yu
author_facet Lee, Shou-Lun
Hsu, Wei-Hsiang
Tu, Chia-Ming
Wang, Wen-Han
Yang, Cheng-Yao
Lee, Hsien-Kuang
Chin, Ting-Yu
author_sort Lee, Shou-Lun
collection PubMed
description BACKGROUND: The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. METHODS: The aHSCs used in this study were derived from hepatic stellate cells that were isolated and purified from the livers of male Wistar rats and then transformed into the activated phenotype by culturing on uncoated plastic dishes. Freshwater clam extract (CE) was collected after the outflow from the live freshwater clams in a water bath at 100°C for 60 min. The effects of CE on aHSCs were analyzed by MTT assay, flow cytometry, Oil Red O (ORO) staining, western blot, and real-time RT-PCR. RESULTS: The results indicated that CE suppressed the proliferation of aHSCs through G0/G1 cell cycle arrest by downregulating cyclin D1 and upregulating p27. The expression levels of a-SMA, collagen I, TGF-β, and TNF-α were inhibited in the CE-treated aHSCs. In addition, the CE treatment increased the lipid contents in aHSCs by promoting PPARγ expression. Furthermore, CE modulated the expression of ECM-related genes, i.e., by upregulating MMP-9 and downregulating TIMP-II. CONCLUSIONS: These data revealed that CE could induce the deactivation of aHSCs. We therefore suggest that CE has potential as an adjuvant therapeutic agent against hepatic fibrosis.
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spelling pubmed-86045812021-11-20 The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells Lee, Shou-Lun Hsu, Wei-Hsiang Tu, Chia-Ming Wang, Wen-Han Yang, Cheng-Yao Lee, Hsien-Kuang Chin, Ting-Yu Evid Based Complement Alternat Med Research Article BACKGROUND: The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. METHODS: The aHSCs used in this study were derived from hepatic stellate cells that were isolated and purified from the livers of male Wistar rats and then transformed into the activated phenotype by culturing on uncoated plastic dishes. Freshwater clam extract (CE) was collected after the outflow from the live freshwater clams in a water bath at 100°C for 60 min. The effects of CE on aHSCs were analyzed by MTT assay, flow cytometry, Oil Red O (ORO) staining, western blot, and real-time RT-PCR. RESULTS: The results indicated that CE suppressed the proliferation of aHSCs through G0/G1 cell cycle arrest by downregulating cyclin D1 and upregulating p27. The expression levels of a-SMA, collagen I, TGF-β, and TNF-α were inhibited in the CE-treated aHSCs. In addition, the CE treatment increased the lipid contents in aHSCs by promoting PPARγ expression. Furthermore, CE modulated the expression of ECM-related genes, i.e., by upregulating MMP-9 and downregulating TIMP-II. CONCLUSIONS: These data revealed that CE could induce the deactivation of aHSCs. We therefore suggest that CE has potential as an adjuvant therapeutic agent against hepatic fibrosis. Hindawi 2021-11-12 /pmc/articles/PMC8604581/ /pubmed/34804181 http://dx.doi.org/10.1155/2021/6065168 Text en Copyright © 2021 Shou-Lun Lee et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Shou-Lun
Hsu, Wei-Hsiang
Tu, Chia-Ming
Wang, Wen-Han
Yang, Cheng-Yao
Lee, Hsien-Kuang
Chin, Ting-Yu
The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_full The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_fullStr The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_full_unstemmed The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_short The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_sort effects of freshwater clam (corbicula fluminea) extract on activated hepatic stellate cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604581/
https://www.ncbi.nlm.nih.gov/pubmed/34804181
http://dx.doi.org/10.1155/2021/6065168
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