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Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2

The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based dev...

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Autores principales: Wang, Jiangshan, Dextre, Andres, Pascual-Garrigos, Ana, Davidson, Josiah Levi, Maruthamuthu, Murali K., McChesney, Darby, Seville, Jordan, Verma, Mohit S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604794/
https://www.ncbi.nlm.nih.gov/pubmed/34840962
http://dx.doi.org/10.1016/j.mex.2021.101586
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author Wang, Jiangshan
Dextre, Andres
Pascual-Garrigos, Ana
Davidson, Josiah Levi
Maruthamuthu, Murali K.
McChesney, Darby
Seville, Jordan
Verma, Mohit S.
author_facet Wang, Jiangshan
Dextre, Andres
Pascual-Garrigos, Ana
Davidson, Josiah Levi
Maruthamuthu, Murali K.
McChesney, Darby
Seville, Jordan
Verma, Mohit S.
author_sort Wang, Jiangshan
collection PubMed
description The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based device that uses reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect SARS-CoV-2 in complex matrices by providing a colorimetric response apparent to the naked eye. Because of LAMP's functionality, this device just requires a simple heat source (e.g., water bath, incubator), it can be deployed in resource-constrained areas and can be used as a supplement to current point-of-care (POC) and community testing procedures. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostics, etc. simply by changing the specific primers. • We developed a platform capable of on-paper detection of SARS-CoV-2 using colorimetric reporters that produce responses visible to the naked eye. • The platform is easily reconfigurable to target different pathogens by changing the primer sets, and multiplexing is possible by adding additional reaction sites to the device.
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spelling pubmed-86047942021-11-22 Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2 Wang, Jiangshan Dextre, Andres Pascual-Garrigos, Ana Davidson, Josiah Levi Maruthamuthu, Murali K. McChesney, Darby Seville, Jordan Verma, Mohit S. MethodsX Method Article The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based device that uses reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect SARS-CoV-2 in complex matrices by providing a colorimetric response apparent to the naked eye. Because of LAMP's functionality, this device just requires a simple heat source (e.g., water bath, incubator), it can be deployed in resource-constrained areas and can be used as a supplement to current point-of-care (POC) and community testing procedures. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostics, etc. simply by changing the specific primers. • We developed a platform capable of on-paper detection of SARS-CoV-2 using colorimetric reporters that produce responses visible to the naked eye. • The platform is easily reconfigurable to target different pathogens by changing the primer sets, and multiplexing is possible by adding additional reaction sites to the device. Elsevier 2021-11-20 /pmc/articles/PMC8604794/ /pubmed/34840962 http://dx.doi.org/10.1016/j.mex.2021.101586 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Method Article
Wang, Jiangshan
Dextre, Andres
Pascual-Garrigos, Ana
Davidson, Josiah Levi
Maruthamuthu, Murali K.
McChesney, Darby
Seville, Jordan
Verma, Mohit S.
Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_full Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_fullStr Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_full_unstemmed Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_short Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2
title_sort fabrication of a paper-based colorimetric molecular test for sars-cov-2
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604794/
https://www.ncbi.nlm.nih.gov/pubmed/34840962
http://dx.doi.org/10.1016/j.mex.2021.101586
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